ArticleJournal of bacteriology2006
Nickel represses the synthesis of the nickel permease NixA of Helicobacter pylori.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Therapeutic suppression ofFrontiers in microbiology · 2026Article
- Insights into the Orchestration of Gene Transcription Regulators inInternational journal of molecular sciences · 2022Review
- The Effects of Vitamins and Micronutrients onJournal of immunology research · 2022Review
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Comprehensive mapping of the Helicobacter pylori NikR regulon provides new insights in bacterial nickel responses.Scientific reports · 2017Article
- Characterization in Helicobacter pylori of a Nickel Transporter Essential for Colonization That Was Acquired during Evolution by Gastric Helicobacter Species.PLoS pathogens · 2016Article
- Evolution of Helicobacter: Acquisition by Gastric Species of Two Histidine-Rich Proteins Essential for Colonization.PLoS pathogens · 2015Article
- On the interaction of Helicobacter pylori NikR, a Ni(II)-responsive transcription factor, with the urease operator: in solution and in silico studies.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2015Article
- Crosstalk between the HpArsRS two-component system and HpNikR is necessary for maximal activation of urease transcription.Frontiers in microbiology · 2015Article
- Nickel trafficking system responsible for urease maturation in Helicobacter pylori.World journal of gastroenterology · 2013Review
- Common themes and unique proteins for the uptake and trafficking of nickel, a metal essential for the virulence of Helicobacter pylori.Frontiers in cellular and infection microbiology · 2013Review
- Integrative computational protocol for the discovery of inhibitors of the Helicobacter pylori nickel response regulator (NikR).Journal of molecular modeling · 2011Article
- Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori.Nucleic acids research · 2011Article
- In vivo recognition of the fecA3 target promoter by Helicobacter pylori NikR.Journal of bacteriology · 2011Article
- Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli.Biochemistry · 2010Article
- Built shallow to maintain homeostasis and persistent infection: insight into the transcriptional regulatory network of the gastric human pathogen Helicobacter pylori.PLoS pathogens · 2010Review
- An intact urease assembly pathway is required to compete with NikR for nickel ions in Helicobacter pylori.Journal of bacteriology · 2009Article
- Article
- Nickel-binding and accessory proteins facilitating Ni-enzyme maturation in Helicobacter pylori.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2007Review
- Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nickel acquisition is necessary for urease activity, a major virulence factor of the human gastric pathogen Helicobacter pylori. NixA was identified as a specific nickel uptake system in this organism. Addition of small amounts of nickel to media strongly stimulates urea hydrolysis. On the other hand, high nickel concentrations are deleterious to cell growth. As a possible protective reaction, nickel uptake seems to be reduced in H. pylori grown in nickel-rich media. These observations led to investigations of regulation of the expression of the nickel permease NixA. We found that increasing the nickel concentration in media reduced the amount of NixA. In order to address the question of whether this phenomenon was subject to transcriptional or translational regulation, we quantified nixA mRNA from H. pylori by real-time PCR. The amount of nixA mRNA was gradually reduced five- to sevenfold in a time- and concentration-dependent manner. Repression could be measured as soon as 5 min after nickel addition, and the maximum repression occurred after 20 to 30 min. The maximum repression was obtained with an external nickel concentration of 100 microM. The observed nickel repression of NixA was dependent on nikR encoding the nickel-responsive regulatory protein NikR. In conclusion, we demonstrated that synthesis of the NixA nickel permease of H. pylori shows nickel-responsive regulation mediated by NikR to maintain the balance between effective nickel acquisition and a toxic overload.
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