ArticleGenetics2006
Functions of multiple exonucleases are essential for cell viability, DNA repair and homologous recombination in recD mutants of Escherichia coli.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Single-strand DNA-binding protein suppresses illegitimate recombination in Escherichia coli, acting in synergy with RecQ helicase.Scientific reports · 2024Article
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- Structure ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Still finding ways to augment the existing management of acute and chronic kidney diseases with targeted gene and cell therapies: Opportunities and hurdles.Frontiers in medicine · 2023Review
- Role for DNA double strand end-resection activity of RecBCD in control of aberrant chromosomal replication initiation in Escherichia coli.Nucleic acids research · 2022Article
- Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome.PLoS genetics · 2018Article
- 3'-Terminated Overhangs Regulate DNA Double-Strand Break Processing inG3 (Bethesda, Md.) · 2017Article
- Homologous recombination mediated by the mycobacterial AdnAB helicase without end resection by the AdnAB nucleases.Nucleic acids research · 2017Article
- Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII.Journal of microbiology (Seoul, Korea) · 2015Article
- End resection at double-strand breaks: mechanism and regulation.Cold Spring Harbor perspectives in biology · 2014Review
- The adnAB locus, encoding a putative helicase-nuclease activity, is essential in Streptomyces.Journal of bacteriology · 2014Article
- Structural insights into DNA repair by RNase T--an exonuclease processing 3' end of structured DNA in repair pathways.PLoS biology · 2014Article
- Recognition and processing of double-stranded DNA by ExoX, a distributive 3'-5' exonuclease.Nucleic acids research · 2013Article
- Mealybugs nested endosymbiosis: going into the 'matryoshka' system in Planococcus citri in depth.BMC microbiology · 2013Article
- Delineation of structural domains and identification of functionally important residues in DNA repair enzyme exonuclease VII.Nucleic acids research · 2012Article
- Replication fork reversal after replication-transcription collision.PLoS genetics · 2012Article
- DNA end resection controls the balance between homologous and illegitimate recombination in Escherichia coli.PloS one · 2012Article
- The DNA Exonucleases of Escherichia coli.EcoSal Plus · 2011Article
- RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli.Genetics · 2010Article
- All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W.PloS one · 2010Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterotrimeric RecBCD enzyme unwinds and resects a DNA duplex containing blunt double-stranded ends and directs loading of the strand-exchange protein RecA onto the unwound 3'-ending strand, thereby initiating the majority of recombination in wild-type Escherichia coli. When the enzyme lacks its RecD subunit, the resulting RecBC enzyme, active in recD mutants, is recombination proficient although it has only helicase and RecA loading activity and is not a nuclease. However, E. coli encodes for several other exonucleases that digest double-stranded and single-stranded DNA and thus might act in consort with the RecBC enzyme to efficiently promote recombination reactions. To test this hypothesis, I inactivated multiple exonucleases (i.e., exonuclease I, exonuclease X, exonuclease VII, RecJ, and SbcCD) in recD derivatives of the wild-type and nuclease-deficient recB1067 strain and assessed the ability of the resultant mutants to maintain cell viability and to promote DNA repair and homologous recombination. A complex pattern of overlapping and sometimes competing activities of multiple exonucleases in recD mutants was thus revealed. These exonucleases were shown to be essential for cell viability, DNA repair (of UV- and gamma-induced lesions), and homologous recombination (during Hfr conjugation and P1 transduction), which are dependent on the RecBC enzyme. A model for donor DNA processing in recD transconjugants and transductants was proposed.
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