ArticleGenetics2006
Efficient gene targeting in Drosophila with zinc-finger nucleases.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
109 citing papers in PubMed, 237 citations in OpenAlex.
- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
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- Genetic control of the error-prone repair of a chromosomal double-strand break with 5' overhangs in yeast.Genetics · 2023Article
- Genetic control of the error-prone repair of a chromosomal double-strand break with 5' overhangs in yeast.bioRxiv : the preprint server for biology · 2023Article
- Article
- Review
- Mutagenic repair of a ZFN-induced double-strand break in yeast: Effects of cleavage site sequence and spacer size.DNA repair · 2021Article
- Advances in Agrobacterium transformation and vector design result in high-frequency targeted gene insertion in maize.Plant biotechnology journal · 2021Article
- Can genetic engineering-based methods for gene function identification be eclipsed by genome editing in plants? A comparison of methodologies.Molecular genetics and genomics : MGG · 2021Review
- Recombinational Repair of Nuclease-Generated Mitotic Double-Strand Breaks with Different End Structures in Yeast.G3 (Bethesda, Md.) · 2020Article
- Antioxidant Therapy in Parkinson's Disease: Insights fromAntioxidants (Basel, Switzerland) · 2020Review
- Article
- The Problem of the Low Rates of CRISPR/Cas9-Mediated Knock-ins in Plants: Approaches and Solutions.International journal of molecular sciences · 2019Review
- Ways of improving precise knock-in by genome-editing technologies.Human genetics · 2019Review
- Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Role of Gene Editing in Neurodegenerative Diseases.Cell transplantation · 2018Review
- Gene Editing of HIV-1 Co-receptors to Prevent and/or Cure Virus Infection.Frontiers in microbiology · 2018Review
- New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells.Stem cells international · 2018Review
- Imaging Specific Genomic DNA in Living Cells.Annual review of biophysics · 2016Review
- Origins of Programmable Nucleases for Genome Engineering.Journal of molecular biology · 2016Review
49 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
This report describes high-frequency germline gene targeting at two genomic loci in Drosophila melanogaster, y and ry. In the best case, nearly all induced parents produced mutant progeny; 25% of their offspring were new mutants and most of these were targeted gene replacements resulting from homologous recombination (HR) with a marked donor DNA. The procedure that generates these high frequencies relies on cleavage of the target by designed zinc-finger nucleases (ZFNs) and production of a linear donor in situ. Increased induction of ZFN expression led to higher frequencies of gene targeting, demonstrating the beneficial effect of activating the target. In the absence of a homologous donor DNA, ZFN cleavage led to the recovery of new mutants at three loci-y, ry and bw-through nonhomologous end joining (NHEJ) after cleavage. Because zinc fingers can be directed to a broad range of DNA sequences and targeting is very efficient, this approach promises to allow genetic manipulation of many different genes, even in cases where the mutant phenotype cannot be predicted.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.