Evidence map›Paper›PMID 16450172›Full record

ArticlePlanta2006

Isolation of a cDNA clone (PcSrp) encoding serine-rich-protein from Porteresia coarctata T. and its expression in yeast and finger millet (Eleusine coracana L.) affording salt tolerance.

S Mahalakshmi, G S B Christopher, T P Reddy, K V Rao, V D Reddy

Abstract read
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In one paragraph

Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. The potentialities of omics resources for millet improvement.Functional & integrative genomics · 2023
    Review
  7. Review
  8. Review
  9. Salinity tolerance mechanisms and their breeding implications.Journal, genetic engineering & biotechnology · 2021
    Review
  10. Review
  11. Review
  12. Finger Millet [Frontiers in plant science · 2018
    Review
  13. Article
  14. Gene Discovery and Advances in Finger Millet [Frontiers in plant science · 2016
    Review
  15. Review
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

S MahalakshmiCentre for Plant Molecular Biology, Osmania University, 500 007 Hyderabad, AP, India.
G S B Christopher
T P Reddy
K V Rao
V D Reddy

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A 1.4 Kb cDNA clone encoding a serine-rich protein has been isolated from the cDNA library of salt stressed roots of Porteresia coarctata, and designated as P. coarctata serine-rich-protein (PcSrp) encoding gene. Northern analysis and in situ mRNA hybridization revealed the expression of PcSrp in the salt stressed roots and rhizome of P. coarctata. However, no such expression was seen in the salt stressed leaves and in the unstressed tissues of root, rhizome and leaf, indicating that PcSrp is under the control of a salt-inducible tissue-specific promoter. In yeast, the PcSrp conferred increased NaCl tolerance, implicating its role in salinity tolerance at cellular level. Further, PcSrp was cloned downstream to rice Actin-1 promoter and introduced into finger millet through particle-inflow-gun method. Transgenic plants expressing PcSrp were able to grow to maturity and set seed under 250 mM NaCl stress. The untransformed control plants by contrast failed to survive under similar salt stress. The stressed roots of transgenic plants invariably accumulated higher Na(+) and K(+) ion contents compared to roots of untransformed plants; whereas, shoots of transgenics accumulated lower levels of both the ions than that of untransformed plants under identical stress, clearly suggesting the involvement of PcSrp in ion homeostasis contributing to salt tolerance.

Indexed as

Adaptation, PhysiologicalDNA, ComplementaryEleusineEscherichia coliGene Expression Regulation, PlantGerminationMolecular Sequence DataOryzaPlant ProteinsPlant RootsPlants, Genetically ModifiedPotassiumRNA, MessengerSaccharomyces cerevisiaeSeedsSerineDNA, ComplementaryPlant ProteinsPotassiumRNA, MessengerSerineSodiumSodium Chloride

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.