ArticleJournal of lipid research2006
Agpat6--a novel lipid biosynthetic gene required for triacylglycerol production in mammary epithelium.
Article in Journal of lipid research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 128 citations in OpenAlex.
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- Identifying Residues for Substrate Recognition in Human GPAT4 by Molecular Dynamics Simulations.International journal of molecular sciences · 2024Article
- C/EBPα promotes triacylglycerol synthesis via regulating PPARG promoter activity in goat mammary epithelial cells.Journal of animal science · 2023Article
- Comparative proteomic analysis of human milk fat globules and paired membranes and mouse milk fat globules identifies core cellular systems contributing to mammary lipid trafficking and secretion.Frontiers in molecular biosciences · 2023Article
- Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities.International journal of molecular sciences · 2021Article
- Hormone-sensitive lipase couples intergenerational sterol metabolism to reproductive success.eLife · 2021Article
- Phospholipids containing ether-bound hydrocarbon-chains are essential for efficient phagocytosis and neutral lipids of the ester-type perturb development inBiology open · 2020Article
- Distinguishing pleiotropy from linked QTL between milk production traits and mastitis resistance in Nordic Holstein cattle.Genetics, selection, evolution : GSE · 2020Article
- Epithelial-adipocyte interactions are required for mammary gland development, but not for milk production or fertility.Developmental biology · 2020Article
- Detection of Single-Nucleotide Polymorphism inIranian journal of biotechnology · 2019Article
- It takes a village: channeling fatty acid metabolism and triacylglycerol formation via protein interactomes.Journal of lipid research · 2019Review
- Transcriptional Regulation of Acyl-CoA:Glycerol-International journal of molecular sciences · 2019Review
- Rapid communication: lipid metabolic gene expression and triacylglycerol accumulation in goat mammary epithelial cells are decreased by inhibition of SREBP-1.Journal of animal science · 2018Article
- Update on glycerol-3-phosphate acyltransferases: the roles in the development of insulin resistance.Nutrition & diabetes · 2018Review
- Th-POK regulates mammary gland lactation through mTOR-SREBP pathway.PLoS genetics · 2018Article
- How lipid droplets "TAG" along: Glycerolipid synthetic enzymes and lipid storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest).PloS one · 2017Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
In analyzing the sequence tags for mutant mouse embryonic stem (ES) cell lines in BayGenomics (a mouse gene-trapping resource), we identified a novel gene, 1-acylglycerol-3-phosphate O-acyltransferase (Agpat6), with sequence similarities to previously characterized glycerolipid acyltransferases. Agpat6's closest family member is another novel gene that we have provisionally designated Agpat8. Both Agpat6 and Agpat8 are conserved from plants, nematodes, and flies to mammals. AGPAT6, which is predicted to contain multiple membrane-spanning helices, is found exclusively within the endoplasmic reticulum (ER) in mammalian cells. To gain insights into the in vivo importance of Agpat6, we used the Agpat6 ES cell line from BayGenomics to create Agpat6-deficient (Agpat6-/-) mice. Agpat6-/- mice lacked full-length Agpat6 transcripts, as judged by northern blots. One of the most striking phenotypes of Agpat6-/- mice was a defect in lactation. Pups nursed by Agpat6-/- mothers die perinatally. Normally, Agpat6 is expressed at high levels in the mammary epithelium of breast tissue, but not in the surrounding adipose tissue. Histological studies revealed that the aveoli and ducts of Agpat6-/- lactating mammary glands were underdeveloped, and there was a dramatic decrease in the size and number of lipid droplets within mammary epithelial cells and ducts. Also, the milk from Agpat6-/- mice was markedly depleted in diacylglycerols and triacylglycerols. Thus, we identified a novel glycerolipid acyltransferase of the ER, AGPAT6, which is crucial for the production of milk fat by the mammary gland.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.