ArticleBlood2006
Mechanism of platelet adhesion to von Willebrand factor and microparticle formation under high shear stress.
Article in Blood, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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Who cites it
133 citing papers in PubMed, 330 citations in OpenAlex.
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- Platelet-derived S100A9 contributes to endotheliopathy in alcohol-associated hepatitis.JCI insight · 2026Article
- Von Willebrand factor A1 blockade prevents platelet-mediated sustained occlusion for the treatment of arterial thrombosis.Communications biology · 2026Article
- The Effect of von Willebrand Disease on Platelet Adhesion Dynamics: Correlating a Multiscale Platelet Model to In Vitro Results.IEEE transactions on bio-medical engineering · 2026Article
- Computational and Experimental Assessment of Shear-Induced Blood Trauma by HeartMate II, HeartMate 3, and BrioVAD.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2026Article
- Agonal cell resuscitation strategy to promote tissue repair.Nature communications · 2026Article
- Quantitative Flow Cytometry-Medical Applications with a Focus on Blood Platelets.International journal of molecular sciences · 2026Review
- Platelet-derived integrin- and tetraspanin-enriched tethers exacerbate severe inflammation.Science (New York, N.Y.) · 2026Article
- Hemodynamic consequences of TEVAR with different configurations ofFrontiers in cardiovascular medicine · 2026Article
- Engineering design of platelet-mimicking therapeutic systems: multilevel biomimicry, gating strategies, and translational boundaries.Frontiers in bioengineering and biotechnology · 2026Review
- Reversible formation of von-Willebrand-factor-platelet aggregates in microvascular blood flow.PNAS nexus · 2025Article
- Key markers and detection methods for evaluating platelet dysfunction in mechanical circulatory support devices.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2025Review
- Endo-chip laser-induced thrombus formation: a vessel-on-chip model for in vitro testing of antithrombotic agents.Blood vessels, thrombosis & hemostasis · 2025Article
- The pivotal role of von Willebrand factor binding to platelet αIIbβ3 in stabilizing the formation of a platelet plug at sites of injury.Haematologica · 2025Article
- Proteomics of arterial thrombi in acute limb ischemia.Journal of thrombosis and thrombolysis · 2025Article
- Combination of Polydopamine and Plasma Oxidation to Increase Tissue Integration of Polyurethane-Silicone Copolymers for Cardiovascular Implants.Advanced healthcare materials · 2025Article
- Extracellular vesicles in ageing cold-stored whole blood may not compensate for the decreasing haemostatic function in vitro.Transfusion medicine (Oxford, England) · 2025Article
- Hemodynamic simulation andPhysics of fluids (Woodbury, N.Y. : 1994) · 2025Article
- Freeze-dried plasma: Hemostasis and biophysical analyses for damage control resuscitation.Transfusion · 2025Article
- The Role of HSP47 in Thrombotic Disorders: Molecular Mechanism and Therapeutic Potential.Current issues in molecular biology · 2025Review
73 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We describe here the mechanism of platelet adhesion to immobilized von Willebrand factor (VWF) and subsequent formation of platelet-derived microparticles mediated by glycoprotein Ibalpha (GPIbalpha) under high shear stress. As visualized in whole blood perfused in a flow chamber, platelet attachment to VWF involved one or few membrane areas of 0.05 to 0.1 microm(2) that formed discrete adhesion points (DAPs) capable of resisting force in excess of 160 pN. Under the influence of hydrodynamic drag, membrane tethers developed between the moving platelet body and DAPs firmly adherent to immobilized VWF. Continued stretching eventually caused the separation of many such tethers, leaving on the surface tube-shaped or spherical microparticles with a diameter as low as 50 to 100 nm. Adhesion receptors (GPIbalpha, alphaIIbbeta3) and phosphatidylserine were expressed on the surface of these microparticles, which were procoagulant. Shearing platelet-rich plasma at the rate of 10,000 s(-1) in a cone-and-plate viscosimeter increased microparticle counts up to 55-fold above baseline. Blocking the GPIb-VWF interaction abolished microparticle generation in both experimental conditions. Thus, a biomechanical process mediated by GPIbalpha-VWF bonds in rapidly flowing blood may not only initiate platelet arrest onto reactive vascular surfaces but also generate procoagulant microparticles that further enhance thrombus formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.