ArticleNucleic acids research2006
Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
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- Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications.Tissue engineering. Part A · 2019Review
- Engineering Whole Mammalian Cells for Target-Cell-Specific Invasion/Fusion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Intracellular targeting with engineered proteins.F1000Research · 2016Review
- Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant.Nature communications · 2014Article
- Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases.eLife · 2014Article
- Lentiviral protein transduction with genome-modifying HIV-1 integrase-I-PpoI fusion proteins: studies on specificity and cytotoxicity.BioMed research international · 2014Article
- Driving DNA transposition by lentiviral protein transduction.Mobile genetic elements · 2014Article
- Oncolytic viruses in the treatment of cancer: a review of current strategies.Pathology oncology research : POR · 2012Review
- Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line.In vitro cellular & developmental biology. Animal · 2012Article
- Emerging biomedical applications of synthetic biology.Nature reviews. Genetics · 2011Review
- Efficient gene targeting mediated by a lentiviral vector-associated meganuclease.Nucleic acids research · 2011Article
- On the biomarkers and mechanisms of konzo, a distinct upper motor neuron disease associated with food (cassava) cyanogenic exposure.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2011Article
- Protein transduction from retroviral Gag precursors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch.Nucleic acids research · 2008Article
- A synthetic time-delay circuit in mammalian cells and mice.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The status of gene therapy for brain tumors.Expert opinion on biological therapy · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The straightforward production and dose-controlled administration of protein therapeutics remain major challenges for the biopharmaceutical manufacturing and gene therapy communities. Transgenes linked to HIV-1-derived vpr and pol-based protease cleavage (PC) sequences were co-produced as chimeric fusion proteins in a lentivirus production setting, encapsidated and processed to fusion peptide-free native protein in pseudotyped lentivirions for intracellular delivery and therapeutic action in target cells. Devoid of viral genome sequences, protein-transducing nanoparticles (PTNs) enabled transient and dose-dependent delivery of therapeutic proteins at functional quantities into a variety of mammalian cells in the absence of host chromosome modifications. PTNs delivering Manihot esculenta linamarase into rodent or human, tumor cell lines and spheroids mediated hydrolysis of the innocuous natural prodrug linamarin to cyanide and resulted in efficient cell killing. Following linamarin injection into nude mice, linamarase-transducing nanoparticles impacted solid tumor development through the bystander effect of cyanide.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.