ArticleIn vitro cellular & developmental biology. Animal
Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Establishment of a Serum-Free Human iPSC-Derived Model of Peripheral Myelination.ACS biomaterials science & engineering · 2024Article
- A functional aged human iPSC-cortical neuron model recapitulates Alzheimer's disease, senescence, and the response to therapeutics.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2024Article
- Physiological Aβ Concentrations Produce a More Biomimetic Representation of the Alzheimer's Disease Phenotype in iPSC Derived Human Neurons.ACS chemical neuroscience · 2018Article
- Comparison of NMDA and AMPA Channel Expression and Function between Embryonic and Adult Neurons Utilizing Microelectrode Array Systems.ACS biomaterials science & engineering · 2017Article
- Tissue engineering the mechanosensory circuit of the stretch reflex arc with human stem cells: Sensory neuron innervation of intrafusal muscle fibers.Biomaterials · 2017Article
- A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord.Biomaterials · 2014Article
- In vitro Differentiation of Functional Human Skeletal Myotubes in a Defined System.Biomaterials science · 2014Article
- Two cell circuits of oriented adult hippocampal neurons on self-assembled monolayers for use in the study of neuronal communication in a defined system.ACS chemical neuroscience · 2013Article
- Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1.Biomaterials · 2013Article
- Rat Cortical Oligodendrocyte-Embryonic Motoneuron Co-Culture: AnJournal of biomaterials and tissue engineering · 2012Article
- Small Molecule Induction of Human Umbilical Stem Cells into MBP-positive Oligodendrocytes in a Defined Three-Dimensional Environment.ACS chemical neuroscience · 2012Article
- Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system.Biomaterials · 2011Article
- Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture.Journal of vacuum science and technology. B, Nanotechnology & microelectronics : materials, processing, measurement, & phenomena : JVST B · 2011Article
- Neuromuscular junction formation between human stem-cell-derived motoneurons and rat skeletal muscle in a defined system.Tissue engineering. Part C, Methods · 2010Article
- Coexpression of glutamate vesicular transporter (VGLUT1) and choline acetyltransferase (ChAT) proteins in fetal rat hippocampal neurons in culture.In vitro cellular & developmental biology. Animal · 2010Article
- Addition of glutamate to serum-free culture promotes recovery of electrical activity in adult hippocampal neurons in vitro.Journal of neuroscience methods · 2010Article
- Article
- Characterization of a human fetal spinal cord stem cell line, NSI-566RSC, and its induction to functional motoneurons.Journal of tissue engineering and regenerative medicine · 2010Article
- Tissue engineering intrafusal fibers: dose- and time-dependent differentiation of nuclear bag fibers in a defined in vitro system using neuregulin 1-beta-1.Biomaterials · 2008Article
- Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture.Experimental neurology · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology, the development of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated on a glass surface and an empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3, brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the cultures were maintained for 4-6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional in vitro system.
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