ArticleThe Journal of general physiology2006
A Na+- and Cl- -activated K+ channel in the thick ascending limb of mouse kidney.
Article in The Journal of general physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Review
- Role of calcineurin in regulating renal potassium (KActa physiologica (Oxford, England) · 2024Review
- Inwardly rectifying KAmerican journal of physiology. Cell physiology · 2022Review
- Caloric Restriction and Ketogenic Diet Therapy for Epilepsy: A Molecular Approach InvolvingFrontiers in neurology · 2020Review
- ClAmerican journal of physiology. Cell physiology · 2020Review
- Kir4.1/Kir5.1 in the DCT plays a role in the regulation of renal KAmerican journal of physiology. Renal physiology · 2019Article
- Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.Physiological reviews · 2019Review
- Epithelial transport inThe Journal of general physiology · 2017Review
- The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection.The Biochemical journal · 2017Review
- International Union of Basic and Clinical Pharmacology. C. Nomenclature and Properties of Calcium-Activated and Sodium-Activated Potassium Channels.Pharmacological reviews · 2017Review
- Basolateral Kir4.1 activity in the distal convoluted tubule regulates K secretion by determining NaCl cotransporter activity.Current opinion in nephrology and hypertension · 2016Review
- Differential distribution of the sodium-activated potassium channels slick and slack in mouse brain.The Journal of comparative neurology · 2016Article
- The expression, regulation, and function of Kir4.1 (Kcnj10) in the mammalian kidney.American journal of physiology. Renal physiology · 2016Review
- SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels.The Journal of biological chemistry · 2016Article
- Article
- Vasopressin-induced stimulation of the Na(+)-activated K(+) channels is responsible for maintaining the basolateral K(+) conductance of the thick ascending limb (TAL) in EAST/SeSAME syndrome.Biochimica et biophysica acta · 2015Article
- KCNJ10 (Kir4.1) is expressed in the basolateral membrane of the cortical thick ascending limb.American journal of physiology. Renal physiology · 2015Article
- Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.Physiological reviews · 2015Review
- Intracellular ATP does not inhibit Slo2.1 K+ channels.Physiological reports · 2014Article
- Evidence of K+ channel function in epithelial cell migration, proliferation, and repair.American journal of physiology. Cell physiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigates the presence and properties of Na+-activated K+ (K(Na)) channels in epithelial renal cells. Using real-time PCR on mouse microdissected nephron segments, we show that Slo2.2 mRNA, which encodes for the K(Na) channels of excitable cells, is expressed in the medullary and cortical thick ascending limbs of Henle's loop, but not in the other parts of the nephron. Patch-clamp analysis revealed the presence of a high conductance K+ channel in the basolateral membrane of both the medullary and cortical thick ascending limbs. This channel was highly K+ selective (P(K)/P(Na) approximately 20), its conductance ranged from 140 to 180 pS with subconductance levels, and its current/voltage relationship displayed intermediate, Na+-dependent, inward rectification. Internal Na+ and Cl- activated the channel with 50% effective concentrations (EC50) and Hill coefficients (nH) of 30 +/- 1 mM and 3.9 +/- 0.5 for internal Na+, and 35 +/- 10 mM and 1.3 +/- 0.25 for internal Cl-. Channel activity was unaltered by internal ATP (2 mM) and by internal pH, but clearly decreased when internal free Ca2+ concentration increased. This is the first demonstration of the presence in the epithelial cell membrane of a functional, Na+-activated, large-conductance K+ channel that closely resembles native K(Na) channels of excitable cells. This Slo2.2 type, Na+- and Cl--activated K+ channel is primarily located in the thick ascending limb, a major renal site of transcellular NaCl reabsorption.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.