ArticleThe Journal of general physiology2006
Different transport mechanisms in plant and human AMT/Rh-type ammonium transporters.
Article in The Journal of general physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 94 citations in OpenAlex.
- Rapid Visual Detection ofPlants (Basel, Switzerland) · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- Ammonium transporter expression in sperm of the disease vectorProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- A twin histidine motif is the core structure for high-affinity substrate selection in plant ammonium transporters.The Journal of biological chemistry · 2020Article
- Ammonia Excretion in an Osmoregulatory Syncytium Is Facilitated by AeAmt2, a Novel Ammonia Transporter inFrontiers in physiology · 2018Article
- Ammonia Transporters and Their Role in Acid-Base Balance.Physiological reviews · 2017Review
- Identifying Potential Mechanisms Enabling Acidophily in the Ammonia-Oxidizing Archaeon "Candidatus Nitrosotalea devanaterra".Applied and environmental microbiology · 2016Article
- Health hazards of methylammonium lead iodide based perovskites: cytotoxicity studies.Toxicology research · 2016Article
- GintAMT3 - a Low-Affinity Ammonium Transporter of the Arbuscular Mycorrhizal Rhizophagus irregularis.Frontiers in plant science · 2016Article
- Evolution of Electrogenic Ammonium Transporters (AMTs).Frontiers in plant science · 2016Article
- The rice OsAMT1;1 is a proton-independent feedback regulated ammonium transporter.Plant cell reports · 2015Article
- A nitrogen-dependent switch in the high affinity ammonium transport in Medicago truncatula.Plant molecular biology · 2014Article
- Ammonia transport in the kidney by Rhesus glycoproteins.American journal of physiology. Renal physiology · 2014Review
- Uncoupling of ionic currents from substrate transport in the plant ammonium transporter AtAMT1;2.The Journal of biological chemistry · 2014Article
- Switching substrate specificity of AMT/MEP/ Rh proteins.Channels (Austin, Tex.) · 2014Review
- Ecological significance and complexity of N-source preference in plants.Annals of botany · 2013Article
- Article
- Renal ammonia metabolism and transport.Comprehensive Physiology · 2013Review
- Cystic fibrosis transmembrane conductance regulator in the gills of the climbing perch, Anabas testudineus, is involved in both hypoosmotic regulation during seawater acclimation and active ammonia excretion during ammonia exposure.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conserved family of AMT/Rh proteins facilitates ammonium transport across animal, plant, and microbial membranes. A bacterial homologue, AmtB, forms a channel-like structure and appears to function as an NH3 gas channel. To evaluate the function of eukaryotic homologues, the human RhCG glycoprotein and the tomato plant ammonium transporter LeAMT1;2 were expressed and compared in Xenopus oocytes and yeast. RhCG mediated the electroneutral transport of methylammonium (MeA), which saturated with Km = 3.8 mM at pHo 7.5. Uptake was strongly favored by increasing the pHo and was inhibited by ammonium. Ammonium induced rapid cytosolic alkalinization in RhCG-expressing oocytes. Additionally, RhCG expression was associated with an alkali-cation conductance, which was not significantly permeable to NH4+ and was apparently uncoupled from the ammonium transport. In contrast, expression of the homologous LeAMT1;2 induced pHo-independent MeA+ uptake and specific NH4+ and MeA+ currents that were distinct from endogenous currents. The different mechanisms of transport, including the RhCG-associated alkali-cation conductance, were verified by heterologous expression in appropriate yeast strains. Thus, homologous AMT/Rh-type proteins function in a distinct manner; while LeAMT1;2 carries specifically NH4+, or cotransports NH3/H+, RhCG mediates electroneutral NH3 transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.