ArticleProceedings of the National Academy of Sciences of the United States of America2006
Selection of biocatalysts for chemical synthesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 88 citations in OpenAlex.
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- A growth-based screening strategy for engineering the catalytic activity of an oxygen-sensitive formate dehydrogenase.Applied and environmental microbiology · 2024Article
- Cheating the Cheater: Suppressing False-Positive Enrichment during Biosensor-Guided Biocatalyst Engineering.ACS synthetic biology · 2022Article
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- Domestication of proteins - from evolution to revolution.Microbial biotechnology · 2022Article
- Engineering and application of a biosensor with focused ligand specificity.Nature communications · 2020Article
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Development of a Biosensor for Detection of Benzoic Acid Derivatives inFrontiers in bioengineering and biotechnology · 2019Article
- Genetically encoded biosensors for lignocellulose valorization.Biotechnology for biofuels · 2019Review
- Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion.Protein engineering, design & selection : PEDS · 2018Article
- A systematic optimization of styrene biosynthesis inBiotechnology for biofuels · 2018Article
- Enhanced Metabolite Productivity ofFrontiers in bioengineering and biotechnology · 2018Article
- A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts.Microbial biotechnology · 2017Article
- Functional mining of transporters using synthetic selections.Nature chemical biology · 2016Article
- Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System.Journal of visualized experiments : JoVE · 2016Article
- Synthetic biology to access and expand nature's chemical diversity.Nature reviews. Microbiology · 2016Review
- Transcription factor-based biosensors in biotechnology: current state and future prospects.Applied microbiology and biotechnology · 2016Review
- Synthetic biosensors for precise gene control and real-time monitoring of metabolites.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XylC from Pseudomonas putida), the lacZ-based host responded to the oxidation of benzaldehyde and 2-hydroxybenzaldehyde to the corresponding benzoic acids by forming blue colonies, whereas XylC- cells did not. Similarly, the tetA-based host was able to grow under selective conditions only when equipped with XylC, enabling selection of biocatalytically active cells in inactive populations at frequencies as low as 10(-6).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.