ArticleBiochemistry2006
Exploring the recognition of quadruplex DNA by an engineered Cys2-His2 zinc finger protein.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genome-wide meta-analysis points to CTC1 and ZNF676 as genes regulating telomere homeostasis in humans.Human molecular genetics · 2012Pooled it
- G-Quadruplexes: Structural Diversity and Emerging Roles in Biomolecular Condensation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Differences and Similarities in Protein and Nucleic Acid Structures and Their Biological Interactions.Current issues in molecular biology · 2025Review
- G-quadruplexes promote the motility in MAZ phase-separated condensates to activate CCND1 expression and contribute to hepatocarcinogenesis.Nature communications · 2024Article
- Yeast transcription factor Msn2 binds to G4 DNA.Nucleic acids research · 2023Article
- Deciphering RNA G-quadruplex function during the early steps of HIV-1 infection.Nucleic acids research · 2022Article
- G4-quadruplex-binding proteins: review and insights into selectivity.Biophysical reviews · 2022Review
- Convergent analysis of genome-wide genotyping and transcriptomic data suggests association of zinc finger genes with lithium response in bipolar disorder.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2018Article
- Transcriptome Analysis Showed a Differential Signature between Invasive and Non-invasive Corticotrophinomas.Frontiers in endocrinology · 2017Article
- Multivalent LKγ-PNA oligomers bind to a human telomere DNA G-rich sequence to form quadruplexes.Bioorganic & medicinal chemistry letters · 2015Article
- G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins.Nucleic acids research · 2014Article
- Experimental approaches to identify cellular G-quadruplex structures and functions.Methods (San Diego, Calif.) · 2012Article
- A non-canonical DNA structure is a binding motif for the transcription factor SP1 in vitro.Nucleic acids research · 2012Article
- The evolving world of protein-G-quadruplex recognition: a medicinal chemist's perspective.Biochimie · 2011Review
- Article
- Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression.Nucleic acids research · 2009Article
- Chiral metallo-supramolecular complexes selectively recognize human telomeric G-quadruplex DNA.Nucleic acids research · 2008Article
- Selective recognition of a DNA G-quadruplex by an engineered antibody.Biochemistry · 2008Article
- The relationship of potential G-quadruplex sequences in cis-upstream regions of the human genome to SP1-binding elements.Nucleic acids research · 2008Article
- QGRS Mapper: a web-based server for predicting G-quadruplexes in nucleotide sequences.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have recently described an engineered zinc finger protein (Gq1) that binds with high specificity to the intramolecular G-quadruplex formed by the human telomeric sequence 5'-(GGTTAG)(5)-3', and that inhibits the activity of the enzyme telomerase in vitro. Here we report site-directed mutagenesis, biophysical, and molecular modeling studies that provide new insights into quadruplex recognition by the zinc finger scaffold. We show that any one finger of Gq1 can be replaced with the corresponding finger of Zif268, without significant loss of quadruplex affinity or quadruplex versus duplex discrimination. Replacement of two fingers, with one being finger 2, of Gq1 by Zif268 results in significant impairment of quadruplex recognition and loss of discrimination. Molecular modeling suggests that the zinc fingers of Gq1 can bind to the human parallel-stranded quadruplex structure in a stable arrangement, whereas Zif268-quadruplex models show significantly weaker binding energy. Modeling also suggests that an important role of the key protein finger residues in the Gq1-quadruplex complex is to maintain Gq1 in an optimum conformation for quadruplex recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.