Evidence map›Paper›PMID 16444588›Full record

ArticleMolecular and cellular biochemistry2006

Delta-9-tetrahydrocannabinol protects cardiac cells from hypoxia via CB2 receptor activation and nitric oxide production.

Yelena A Shmist, Igor Goncharov, Maor Eichler, Vladimir Shneyvays, Ahuva Isaac, Zvi Vogel, Asher Shainberg

Abstract read
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In one paragraph

Article in Molecular and cellular biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.

  1. Pooled it
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  12. Article
  13. CB2: a cannabinoid receptor with an identity crisis.British journal of pharmacology · 2010
    Review
  14. Endocannabinoids and the heart.Journal of cardiovascular pharmacology · 2009
    Review
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  16. Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yelena A ShmistFaculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Igor Goncharov
Maor Eichler
Vladimir Shneyvays
Ahuva Isaac
Zvi Vogel
Asher Shainberg
Bar-Ilan University · ILWeizmann Institute of Science · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Delta-9-tetrahydrocannabinol (THC), the major active component of marijuana, has a beneficial effect on the cardiovascular system during stress conditions, but the defence mechanism is still unclear. The present study was designed to investigate the central (CB1) and the peripheral (CB2) cannabinoid receptor expression in neonatal cardiomyoctes and possible function in the cardioprotection of THC from hypoxia. Pre-treatment of cardiomyocytes that were grown in vitro with 0.1 - 10 microM THC for 24 h prevented hypoxia-induced lactate dehydrogenase (LDH) leakage and preserved the morphological distribution of alpha-sarcomeric actin. The antagonist for the CB2 (10 microM), but not CB1 receptor antagonist (10 microM) abolished the protective effect of THC. In agreement with these results using RT-PCR, it was shown that neonatal cardiac cells express CB2, but not CB1 receptors. Involvement of NO in the signal transduction pathway activated by THC through CB2 was examined. It was found that THC induces nitric oxide (NO) production by induction of NO synthase (iNOS) via CB2 receptors. L-NAME (NOS inhibitor, 100 microM) prevented the cardioprotection provided by THC. Taken together, our findings suggest that THC protects cardiac cells against hypoxia via CB2 receptor activation by induction of NO production. An NO mechanism occurs also in the classical pre-conditioning process; therefore, THC probably pre-trains the cardiomyocytes to hypoxic conditions.

Indexed as

ActinsAnalgesics, Non-NarcoticAnimalsAnimals, NewbornDronabinolGliomaHypoxiaL-Lactate DehydrogenaseMyocytes, CardiacNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide Synthase Type IIRatsReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Signal TransductionActinsAnalgesics, Non-NarcoticDronabinolL-Lactate DehydrogenaseNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide Synthase Type IIReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2

Identifiers

PMID16444588
OpenAlexW2040457207

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.