ArticleProceedings of the National Academy of Sciences of the United States of America2006
Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
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Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it, 161 citations in OpenAlex.
- Pooled it
- Article
- Cytolysin A is an intracellularly induced and secreted cytotoxin of typhoidal Salmonella.Nature communications · 2024Article
- Structure and ligand binding in the putative anti-microbial peptide transporter protein, YejA.Microbiology (Reading, England) · 2024Article
- Article
- FromACS omega · 2023Review
- Salmonella enterica serovar Typhi influences inflammation and autophagy in macrophages.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- The Role of Egg Yolk in Modulating the Virulence ofFrontiers in cellular and infection microbiology · 2022Article
- Pathogenic signature of invasive non-typhoidalHuman vaccines & immunotherapeutics · 2020Review
- Genome-wide Analysis of Salmonella enterica serovar Typhi in Humanized Mice Reveals Key Virulence Features.Cell host & microbe · 2019Article
- Single-Cell and Time-Resolved Profiling of Intracellular Salmonella Metabolism in Primary Human Cells.Analytical chemistry · 2019Article
- Virulotyping of Salmonella enterica serovar Typhi isolates from Pakistan: Absence of complete SPI-10 in Vi negative isolates.PLoS neglected tropical diseases · 2018Article
- Functional Analysis of the Chaperone-Usher Fimbrial Gene Clusters ofFrontiers in cellular and infection microbiology · 2018Article
- Bi-valent polysaccharides of Vi capsular and O9 O-antigen in attenuatedNPJ vaccines · 2018Article
- The ABC transporter YejABEF is required for resistance to antimicrobial peptides and the virulence of Brucella melitensis.Scientific reports · 2016Article
- A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing.PloS one · 2016Article
- Comparative Analysis of Xenorhabdus koppenhoeferi Gene Expression during Symbiotic Persistence in the Host Nematode.PloS one · 2016Article
- Global transcriptome and mutagenic analyses of the acid tolerance response of Salmonella enterica serovar Typhimurium.Applied and environmental microbiology · 2015Article
- stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells.Biological research · 2015Article
- RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium.PLoS pathogens · 2015Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The cDNA obtained by selective capture of transcribed sequences is a complex mixture that can be used in conjunction with microarrays to determine global gene expression by a pathogen during infection. We used this method to study genes expressed by Salmonella enterica serovar Typhi, the etiological agent of typhoid fever, within human macrophages. Global expression profiles of Typhi grown in vitro and within macrophages at different time points were obtained and compared. Known virulence factors, such as the SPI-1- and SPI-2-encoded type III secretion systems, were found to be expressed as predicted during infection by Salmonella, which validated our data. Typhi inside macrophages showed increased expression of genes encoding resistance to antimicrobial peptides, used the glyoxylate bypass for fatty acid utilization, and did not induce the SOS response or the oxidative stress response. Genes coding for the flagellar apparatus, chemotaxis, and iron transport systems were down-regulated in vivo. Many cDNAs corresponding to genes with unknown functions were up-regulated inside human macrophages and will be important to consider for future studies to elucidate the intracellular lifestyle of this human-specific pathogen. Real-time quantitative PCR was consistent with the microarray results. The combined use of selective capture of transcribed sequences and microarrays is an effective way to determine the bacterial transcriptome in vivo and could be used to investigate transcriptional profiles of other bacterial pathogens without the need to recover many nanograms of bacterial mRNA from host and without increasing the multiplicity of infection beyond what is seen in nature.
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