ArticleDevelopmental and comparative immunology2006
In vivo and in vitro knockdown of FREP2 gene expression in the snail Biomphalaria glabrata using RNA interference.
Article in Developmental and comparative immunology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Snail 3G: genomics, genetics, and gene-editing ofFrontiers in genetics · 2026Review
- CRISPR/Cas9-germline editing ofScience advances · 2025Article
- The chromosome-level genome assembly of the slug Deroceras laeve facilitates its use as a comparative model of regeneration.G3 (Bethesda, Md.) · 2025Article
- Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.Marine biotechnology (New York, N.Y.) · 2025Article
- Article
- A genome sequence for Biomphalaria pfeifferi, the major vector snail for the human-infecting parasite Schistosoma mansoni.PLoS neglected tropical diseases · 2023Article
- Genome of the ramshorn snail Biomphalaria straminea-an obligate intermediate host of schistosomiasis.GigaScience · 2022Article
- Single-cell RNA-seq profiling of individual Biomphalaria glabrata immune cells with a focus on immunologically relevant transcripts.Immunogenetics · 2022Article
- Identification and localization of a gonadotropin-releasing hormone-related neuropeptide in Biomphalaria, an intermediate host for schistosomiasis.The Journal of comparative neurology · 2021Article
- An Overview of Transcriptional Responses of Schistosome-Susceptible (M line) or -Resistant (BS-90)Frontiers in immunology · 2021Article
- Article
- Regulatory effect of heat shock transcription factor-1 gene on heat shock proteins and its transcriptional regulation analysis in small abalone Haliotis diversicolor.BMC molecular and cell biology · 2020Article
- Genomic and transcriptional analysis of genes containing fibrinogen and IgSF domains in the schistosome vector Biomphalaria glabrata, with emphasis on the differential responses of snails susceptible or resistant to Schistosoma mansoni.PLoS neglected tropical diseases · 2020Article
- Immunity-related genes and signaling pathways under hypoxic stresses in Haliotis diversicolor: a transcriptome analysis.Scientific reports · 2019Article
- Gene drives for schistosomiasis transmission control.PLoS neglected tropical diseases · 2019Review
- Signatures of Divergence, Invasiveness, and Terrestrialization Revealed by Four Apple Snail Genomes.Molecular biology and evolution · 2019Article
- Allelic variation in a single genomic region alters the hemolymph proteome in the snail Biomphalaria glabrata.Fish & shellfish immunology · 2019Article
- Sympatric versus allopatric evolutionary contexts shape differential immune response in Biomphalaria / Schistosoma interaction.PLoS pathogens · 2019Article
- Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line.Parasites & vectors · 2018Article
- Allelic variation partially regulates galactose-dependent hydrogen peroxide release from circulating hemocytes of the snail Biomphalaria glabrata.Fish & shellfish immunology · 2018Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
RNA interference (RNAi) is reported here for the first time for Biomphalaria glabrata, the snail intermediate host for the human parasite Schistosoma mansoni. The fibrinogen-related protein 2 (FREP2) gene, normally expressed at increased levels following exposure to digenetic trematode parasites, such as S. mansoni or Echinostoma paraensei, was targeted for knockdown. Double-stranded RNA (dsRNA) corresponding to specific regions of the FREP2 gene was introduced into snails by direct injection into the hemolymph, 2 days prior to exposure to trematodes, or added to co-cultures of B. glabrata embryonic (Bge) cells and E. paraensei sporocysts. After introduction of FREP2 dsRNA, expression levels of FREP2 were significantly reduced, to 20-30% of control values. In addition, we were able to disrupt expression of the house-keeping myoglobin gene, further confirming the feasibility of RNAi for B. glabrata. Cross-reactivity in RNAi has not been observed either among four FREP gene subfamilies or between FREP2 and myoglobin. Establishment of RNAi techniques in B. glabrata provides an important tool for clarifying the function of genes believed to play a role in host-parasite interactions, specifically between B. glabrata and its trematode parasites, including S. mansoni.
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