ArticleThe Journal of physiology2006
Synaptic vesicles in rat hippocampal boutons recycle to different pools in a use-dependent fashion.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Novel pH-Sensitive Lipid Based Exo-Endocytosis Tracers Reveal Fast Intermixing of Synaptic Vesicle Pools.Frontiers in cellular neuroscience · 2018Article
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.The Journal of physiology · 2015Article
- Article
- Multiple dileucine-like motifs direct VGLUT1 trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Sleep and the single neuron: the role of global slow oscillations in individual cell rest.Nature reviews. Neuroscience · 2013Review
- Influence of synaptic vesicle position on release probability and exocytotic fusion mode.Science (New York, N.Y.) · 2012Article
- A readily retrievable pool of synaptic vesicles.Nature neuroscience · 2011Article
- Differential regulation of spontaneous and evoked neurotransmitter release at central synapses.Current opinion in neurobiology · 2011Review
- Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.Neuroscience · 2010Article
- Fluctuation analysis of tetanic rundown (short-term depression) at a corticothalamic synapse.Biophysical journal · 2009Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Activity-dependent control of slow synaptic vesicle endocytosis by cyclin-dependent kinase 5.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Fast synaptic vesicle reuse slows the rate of synaptic depression in the CA1 region of hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- BDNF increases release probability and the size of a rapidly recycling vesicle pool within rat hippocampal excitatory synapses.The Journal of physiology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient vesicle membrane recycling at presynaptic terminals is pivotal for preventing depletion and maintaining high firing rates in neuronal networks. We used a new approach, based on the combination of spectrally different optical probes, to investigate how stimulation determines the fate of synaptic vesicles after endocytosis. We found that in the small central synapses of rat hippocampal neurones low frequency stimulation (40 action potentials at 2 Hz) targets vesicles preferentially to vesicle pools that were kinetically faster. Vesicles taken up during endocytosis triggered by high frequency stimulation (400 action potentials, 20 Hz) were also placed in the back of the release queue. We performed a spatial analysis of the recycled vesicles in living hippocampal boutons using two spectrally different FM-dyes (FM1-43 and FM5-95). By using these consecutively, vesicles endocytosed by either stimulation protocol were labelled with a different colour. This revealed that the kinetic arrangement was also reflected in the spatial organization of vesicles within the bouton. Next, we identified the postsynaptic site of the active zone by transfecting the neurones with postsynaptic density protein PSD-95-CFP. The data from these triple colour experiments suggest that retrieval after low frequency stimulation keeps vesicles in a more confined region closer to the active zone as identified by PSD-95-CFP expression at the postsynaptic site.
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