ArticleBMC genomics2006
Evidence for conservation and selection of upstream open reading frames suggests probable encoding of bioactive peptides.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 94 citations in OpenAlex.
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- Identification of Arabidopsis thaliana upstream open reading frames encoding peptide sequences that cause ribosomal arrest.Nucleic acids research · 2017Article
- Decoding sORF translation - from small proteins to gene regulation.RNA biology · 2016Review
- Separating the wheat from the chaff: systematic identification of functionally relevant noncoding variants in ADHD.Molecular psychiatry · 2016Article
- Extensive identification and analysis of conserved small ORFs in animals.Genome biology · 2015Article
- The generation of knock-in mice expressing fluorescently tagged galanin receptors 1 and 2.Molecular and cellular neurosciences · 2015Article
- Identification of novel Arabidopsis thaliana upstream open reading frames that control expression of the main coding sequences in a peptide sequence-dependent manner.Nucleic acids research · 2015Article
- Identifying (non-)coding RNAs and small peptides: challenges and opportunities.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApproximately 40% of mammalian mRNA sequences contain AUG trinucleotides upstream of the main coding sequence, with a quarter of these AUGs demarcating open reading frames of 20 or more codons. In order to investigate whether these open reading frames may encode functional peptides, we have carried out a comparative genomic analysis of human and mouse mRNA 'untranslated regions' using sequences from the RefSeq mRNA sequence database.
resultsWe have identified over 200 upstream open reading frames which are strongly conserved between the human and mouse genomes. Consensus sequences associated with efficient initiation of translation are overrepresented at the AUG trinucleotides of these upstream open reading frames, while comparative analysis of their DNA and putative peptide sequences shows evidence of purifying selection.
conclusionThe occurrence of a large number of conserved upstream open reading frames, in association with features consistent with protein translation, strongly suggests evolutionary maintenance of the coding sequence and indicates probable functional expression of the peptides encoded within these upstream open reading frames.
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