ArticleWorld journal of gastroenterology2005
Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Corrigendum: Inflammatory and Microbiota-Related Regulation of the Intestinal Epithelial Barrier.Frontiers in nutrition · 2021Article
- Inflammatory and Microbiota-Related Regulation of the Intestinal Epithelial Barrier.Frontiers in nutrition · 2021Review
- Vitamin D3 Versus Gliadin: A Battle to the Last Tight Junction.Digestive diseases and sciences · 2018Article
- Non celiac gluten sensitivity and diagnostic challenges.Gastroenterology and hepatology from bed to bench · 2018Review
- Intestinal epithelium, intraepithelial lymphocytes and the gut microbiota - Key players in the pathogenesis of celiac disease.World journal of gastroenterology · 2017Review
- Overexpression of Hsp70 confers cytoprotection during gliadin exposure in Caco-2 cells.Pediatric research · 2015Article
- Non-celiac gluten sensitivity: Time for sifting the grain.World journal of gastroenterology · 2015Review
- Non-celiac gluten sensitivity: questions still to be answered despite increasing awareness.Cellular & molecular immunology · 2013Review
- New understanding of gluten sensitivity.Nature reviews. Gastroenterology & hepatology · 2012Review
- Divergence of gut permeability and mucosal immune gene expression in two gluten-associated conditions: celiac disease and gluten sensitivity.BMC medicine · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns.
methodsWe treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence) and the tight-junction protein occludin and zonula occluden-1.
resultsThe treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin filaments, impaired and disassembled the integrity of the tight-junction system.
conclusionOur data obtained from an "in vivo-like" polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.