ArticleWorld journal of gastroenterology2005
Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- In Vitro Sensitivity of Neuroendocrine Neoplasms to an Armed Oncolytic Measles Vaccine Virus.Cancers · 2024Article
- A Three-Dimensional Organoid Model of Primary Breast Cancer to Investigate the Effects of Oncolytic Virotherapy.Frontiers in molecular biosciences · 2022Article
- Dental Pulp Stem Cells Derived From Adult Human Third Molar Tooth: A Brief Review.Frontiers in cell and developmental biology · 2021Review
- Suicide gene‑armed measles vaccine virus for the treatment of AML.International journal of oncology · 2019Article
- Cytotoxic response of 5-fluorouracil-resistant cells to gene- and cell-directed enzyme/prodrug treatment.Cancer gene therapy · 2018Article
- The Antitumor Efficacy of a Complex Based on Two-Vector System for Coexpression of the Suicide Gene Fcu1 and Cre Recombinase.Doklady. Biochemistry and biophysics · 2018Article
- Advances in the mechanisms of action of cancer-targeting oncolytic viruses.Oncology letters · 2018Article
- Combination of the oral histone deacetylase inhibitor resminostat with oncolytic measles vaccine virus as a new option for epi-virotherapeutic treatment of hepatocellular carcinoma.Molecular therapy oncolytics · 2015Article
- An armed oncolytic measles vaccine virus eliminates human hepatoma cells independently of apoptosis.Gene therapy · 2013Article
- A novel armed oncolytic measles vaccine virus for the treatment of cholangiocarcinoma.Human gene therapy · 2013Article
- Innate immune defense defines susceptibility of sarcoma cells to measles vaccine virus-based oncolysis.Journal of virology · 2013Article
- Suicidal gene therapy in the effective control of primary human hepatocellular carcinoma as monitored by noninvasive bioimaging.Gene therapy · 2012Article
- Comparative analysis of enzyme and pathway engineering strategies for 5FC-mediated suicide gene therapy applications.Cancer gene therapy · 2011Article
- Promoters with cancer cell-specific activity for melanoma gene therapy.Acta naturae · 2011Article
- Novel strong tissue specific promoter for gene expression in human germ cells.BMC biotechnology · 2010Article
- Targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay.Clinical cancer research : an official journal of the American Association for Cancer Research · 2010Article
- Bystander or no bystander for gene directed enzyme prodrug therapy.Molecules (Basel, Switzerland) · 2009Review
- Abrogation of microsatellite-instable tumors using a highly selective suicide gene/prodrug combination.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- In vitro gene targeting in human hepatoblastoma.Pediatric surgery international · 2006Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.
methodsTo increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.
resultsIn vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH cells stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin (MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application, whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>> YCD>>BCD>>>negative control) was defined as a result of a direct in vivo comparison of all three suicide genes.
conclusionBifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model, thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine.
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