ArticleVeterinary research communications2006
A multiplex PCR to identify porcine mycoplasmas present in broth cultures.
Article in Veterinary research communications, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Antimicrobial susceptibility and genetic profile of Mycoplasma hyopneumoniae isolates from Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Article
- Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method.Journal of veterinary science · 2017Article
- Two clinical isolates ofJournal of veterinary science · 2016Article
- An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis.The Journal of veterinary medical science · 2016Article
- Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay.Journal of clinical microbiology · 2014Article
- Multiple-locus variable-number tandem-repeat analysis is a suitable tool for differentiation of Mycoplasma hyopneumoniae strains without cultivation.Journal of clinical microbiology · 2011Article
- In-depth global analysis of transcript abundance levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus.Advances in virology · 2010Article
- Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection.Veterinary journal (London, England : 1997) · 2009Review
- Role of Toll-like receptors in activation of porcine alveolar macrophages by porcine reproductive and respiratory syndrome virus.Clinical and vaccine immunology : CVI · 2009Article
- Biological enrichment of Mycoplasma agents by cocultivation with permissive cell cultures.Applied and environmental microbiology · 2008Article
- Real-time PCR assays to address genetic diversity among strains of Mycoplasma hyopneumoniae.Journal of clinical microbiology · 2008Article
- Evaluation of tRNA gene PCR for identification of mollicutes.Journal of clinical microbiology · 2005Article
- In vitro susceptibilities of Mycoplasma hyopneumoniae field isolates.Antimicrobial agents and chemotherapy · 2004Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma flocculare can be present in the lungs of pigs at the same time. These three mycoplasma species all require similar growth conditions and can be recovered from clinical samples using the same media. We have developed a multiplex PCR as a helpful tool for rapid differentiation of these three species in the course of isolation. Based on the 16S ribosomal DNA sequences, three different forward primers and a single reverse primer were selected. Each forward primer was compared to available mycoplasma sequences, showing the primers to be specific. The three amplification products observed of 1129 bp (M. hyorhinis), 1000 bp (M. hyopneumoniae) and 754 bp (M. flocculare) were clearly distinguishable on a 1% agarose gel. In addition, no cross-reaction with Mycoplasma hyosynoviae, another porcine mycoplasma, was noted. This multiplex PCR using the proposed set of primers is the first reported assay that allows the simultaneous identification of the different Mycoplasma species isolated from the lungs of pigs.
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