ArticleAntimicrobial agents and chemotherapy2006
Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 74 citations in OpenAlex.
- Physiological cost of antibiotic resistance: Insights from a ribosome variant in bacteria.Science advances · 2024Article
- The point mutation A1387G in the 16S rRNA gene confers aminoglycoside resistance inAntimicrobial agents and chemotherapy · 2024Article
- Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor.Nucleic acids research · 2024Article
- Targeted A-to-G base editing in the organellar genomes of Arabidopsis with monomeric programmable deaminases.Plant physiology · 2024Article
- Fitness Costs of Antibiotic Resistance Impede the Evolution of Resistance to Other Antibiotics.ACS infectious diseases · 2023Article
- The In Vitro Antimicrobial Susceptibility ofPathogens (Basel, Switzerland) · 2023Review
- Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.PLoS biology · 2023Article
- Spectinomycin resistance inFrontiers in microbiology · 2022Article
- A CRISPR interference platform for selective downregulation of gene expression inApplied and environmental microbiology · 2021Article
- A GC-Rich Prophage-Like Genomic Region of Mycoplasma bovirhinis HAZ141_2 Carries a Gene Cluster Encoding Resistance to Kanamycin and Neomycin.Antimicrobial agents and chemotherapy · 2021Article
- Improved bacterial recombineering by parallelized protein discovery.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Pervasive and diverse collateral sensitivity profiles inform optimal strategies to limit antibiotic resistance.PLoS biology · 2019Article
- Aminoglycoside Revival: Review of a Historically Important Class of Antimicrobials Undergoing Rejuvenation.EcoSal Plus · 2018Review
- RNase III Processing of rRNA in the Lyme Disease Spirochete Borrelia burgdorferi.Journal of bacteriology · 2018Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- Functional metagenomic approach to identify overlooked antibiotic resistance mutations in bacterial rRNA.Scientific reports · 2018Article
- Genetic Transformation and Complementation.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report.International medical case reports journal · 2016Article
- The fitness costs of antibiotic resistance mutations.Evolutionary applications · 2015Review
- Genomic analysis reveals distinct concentration-dependent evolutionary trajectories for antibiotic resistance in Escherichia coli.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
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4 authors at 1 institution in 1 country.
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Abstract
We have isolated and characterized in vitro mutants of the Lyme disease agent Borrelia burgdorferi that are resistant to spectinomycin, kanamycin, gentamicin, or streptomycin, antibiotics that target the small subunit of the ribosome. 16S rRNA mutations A1185G and C1186U, homologous to Escherichia coli nucleotides A1191 and C1192, conferred >2,200-fold and 1,300-fold resistance to spectinomycin, respectively. A 16S rRNA A1402G mutation, homologous to E. coli A1408, conferred >90-fold resistance to kanamycin and >240-fold resistance to gentamicin. Two mutations were identified in the gene for ribosomal protein S12, at a site homologous to E. coli residue Lys-87, in mutants selected in streptomycin. Substitutions at codon 88, K88R and K88E, conferred 7-fold resistance and 10-fold resistance, respectively, to streptomycin on B. burgdorferi. The 16S rRNA A1185G and C1186U mutations, associated with spectinomycin resistance, appeared in a population of B. burgdorferi parental strain B31 at a high frequency of 6 x 10(-6). These spectinomycin-resistant mutants successfully competed with the wild-type strain during 100 generations of coculture in vitro. The aminoglycoside-resistant mutants appeared at a frequency of 3 x 10(-9) to 1 x10(-7) in a population and were unable to compete with wild-type strain B31 after 100 generations. This is the first description of mutations in the B. burgdorferi ribosome that confer resistance to antibiotics. These results have implications for the evolution of antibiotic resistance, because the 16S rRNA mutations conferring spectinomycin resistance have no significant fitness cost in vitro, and for the development of new selectable markers.
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