ArticleThe Journal of molecular diagnostics : JMD2006
Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- High resolution melting assay as a reliable method for diagnosing drug-resistant TB cases: a systematic review and meta-analysis.BMC infectious diseases · 2021Pooled it
- High-Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene.Journal of clinical laboratory analysis · 2015Article
- Advances in alloimmune thrombocytopenia: perspectives on current concepts of human platelet antigens, antibody detection strategies, and genotyping.Blood transfusion = Trasfusione del sangue · 2015Review
- Comparison of a simple-probe real-time PCR and multiplex PCR techniques for HPA-1 to HPA-6 and HPA-15 genotyping.Journal of clinical laboratory analysis · 2015Article
- Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.Parasites & vectors · 2014Article
- Human platelet antigen alleles in 998 Taiwanese blood donors determined by sequence-specific primer polymerase chain reaction.BioMed research international · 2013Article
- High-resolution melting analysis as a powerful tool to discriminate and genotype Pseudomonas savastanoi pathovars and strains.PloS one · 2012Article
- A base-calling algorithm for Tm-shifted melting curve SNP assay.Journal of clinical bioinformatics · 2011Article
- Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.Nucleic acids research · 2011Article
- Nucleotide extension genotyping by high-resolution melting.The Journal of molecular diagnostics : JMD · 2010Article
- LightCycler technology in molecular diagnostics.The Journal of molecular diagnostics : JMD · 2009Article
- High resolution melting applications for clinical laboratory medicine.Experimental and molecular pathology · 2008Review
- Rapid diagnosis of MEN2B using unlabeled probe melting analysis and the LightCycler 480 instrument.The Journal of molecular diagnostics : JMD · 2008Article
- Rapid, single-subject genotyping to predict red blood cell antigen expression.Immunohematology · 2008Article
- Characterization of aberrant melting peaks in unlabeled probe assays.The Journal of molecular diagnostics : JMD · 2007Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution melting techniques are a simple and cost-effective alternative to other closed-tube genotyping methods. Here, we genotyped human platelet antigens (HPAs) 1 to 6 and 15 by high-resolution melting methods that did not require labeled probes. Conventional melting analysis with hybridization probes (HybProbes) was also performed at each locus. HybProbe assays were performed individually, whereas amplicon melting (HPAs 1 to 5 and 16) and unlabeled probe (HPA 6) assays were duplexed when possible. At all loci for each method, both homozygous and heterozygous genotypes were easily identified. We analyzed 100 blinded clinical samples (33 amniotic fluid, 12 cultured amniocytes, and 55 blood samples) for all 7 single-nucleotide polymorphisms (SNPs) by each method. Genotype assignments could be made in 99.0% of the SNPs by high-resolution melting and in 98.7% of the SNPs with HybProbes with an overall genotype concordance of 98.8%. Errors included two sample misidentifications and six incorrect assignments that were all resolved by repeating the analysis. Advantages of high-resolution melting include rapid assay development and execution, no need for modified oligonucleotides, and similar accuracy in genotyping compared with other closed-tube melting methods.
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