ArticleThe Journal of molecular diagnostics : JMD2006
Multiplexed detection of anthrax-related toxin genes.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health.Applied and environmental microbiology · 2009Pooled it
- ThermoPlex: an automated design tool for target-specific multiplex PCR primers based on DNA thermodynamics.Biology methods & protocols · 2025Article
- Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides.International journal of molecular sciences · 2021Article
- Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection ofVeterinary sciences · 2015Article
- Swab protocol for rapid laboratory diagnosis of cutaneous anthrax.Journal of clinical microbiology · 2012Article
- Highly specific unnatural base pair systems as a third base pair for PCR amplification.Nucleic acids research · 2012Article
- Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification.BMC microbiology · 2010Article
- Highly sensitive and quantitative detection of the H274Y oseltamivir resistance mutation in seasonal A/H1N1 influenza virus.Journal of clinical microbiology · 2010Article
- Detection and quantification of hepatitis C virus (HCV) by MultiCode-RTx real-time PCR targeting the HCV 3' untranslated region.Journal of clinical microbiology · 2009Article
- Optimization of an unnatural base pair toward natural-like replication.Journal of the American Chemical Society · 2009Article
- Detection of cytomegalovirus in whole blood using three different real-time PCR chemistries.The Journal of molecular diagnostics : JMD · 2009Article
- Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.Current genomics · 2007Article
- Comprehensive DNA signature discovery and validation.PLoS computational biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Simultaneous analysis of three targets in three colors on any real-time polymerase chain reaction (PCR) instrument would increase the flexibility of real-time PCR. For the detection of Bacillus strains that can cause inhalation anthrax-related illness, this ability would be valuable because two plasmids confer virulence, and internal positive controls are needed to monitor the testing in cases lacking target-specific signals. Using a real-time PCR platform called MultiCode-RTx, multiple assays were developed that specifically monitor the presence of Bacillus anthracis-specific virulence plasmid-associated genes. In particular for use on LightCycler-1, two triplex RTx systems demonstrated high sensitivity with limits of detection nearing single-copy levels for both plasmids. Specificity was established using a combination of Ct values and correct amplicon melting temperatures. All reactions were further verified by detection of an internal positive control. For these two triplex RTx assays, the analytical detection limit was one to nine plasmid copy equivalents, 100% analytical specificity with a 95% confidence interval (CI) of 9%, and 100% analytical sensitivity with a CI of 2%. Although further testing using clinical or environmental samples will be required to assess diagnostic sensitivity and specificity, the RTx platform achieves similar results to those of probe-based real-time systems.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.