ArticleThe Journal of molecular diagnostics : JMD2006
Prognostic gene expression signatures can be measured in tissues collected in RNAlater preservative.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Observational
- Article
- Your Blood is Out for Delivery: Considerations of Shipping Time and Temperature on Degradation of RNA from Stabilized Whole Blood.Analytical chemistry · 2025Article
- Your Blood is Out for Delivery: Considerations of Shipping Time and Temperature on Degradation of RNA from Stabilized Whole Blood.bioRxiv : the preprint server for biology · 2024Article
- Response of Total (DNA) and Metabolically Active (RNA) Microbial Communities inMicrobiology spectrum · 2022Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions.Scientific reports · 2019Article
- Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.BMC microbiology · 2019Article
- Nonrandom RNAseq gene expression associated with RNAlater and flash freezing storage methods.Molecular ecology resources · 2019Article
- Sparse quadratic classification rules via linear dimension reduction.Journal of multivariate analysis · 2019Article
- Article
- The role of the pathology department in the preanalytical phase of molecular analyses.Cancer management and research · 2018Article
- RGIFE: a ranked guided iterative feature elimination heuristic for the identification of biomarkers.BMC bioinformatics · 2017Article
- Article
- Functional networks inference from rule-based machine learning models.BioData mining · 2016Article
- Banking placental tissue: an optimized collection procedure for genome-wide analysis of nucleic acids.Placenta · 2014Article
- Up-regulation of neutrophil gelatinase-associated lipocalin in colorectal cancer predicts poor patient survival.World journal of surgery · 2014Article
- Biopsies: next-generation biospecimens for tailoring therapy.Nature reviews. Clinical oncology · 2013Review
- Polyisoprenylated methylated protein methyl esterase is both sensitive to curcumin and overexpressed in colorectal cancer: implications for chemoprevention and treatment.BioMed research international · 2013Article
- ICGE: an R package for detecting relevant clusters and atypical units in gene expression.BMC bioinformatics · 2012Article
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9 authors.
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Abstract
Gene expression signatures have the ability to serve in both prognostic and predictive capacities in patient management. The use of RNA as the starting material and the lability of this analyte, however, dictate that tissues must be snap-frozen or stored in a solution that can maintain the integrity of the RNA. We compared pairs of snap-frozen and RNAlater preservative-suspended tissue from 30 such paired lymph node-negative breast tumors and 21 such paired Dukes' B colon tumors. We assessed the correlation of gene expression profiles and prediction of recurrence based on two prognostic algorithms. Tissues stored in RNAlater preservative generated expression profiles with excellent correlation (average Pearson correlation coefficients of 0.97 and 0.94 for the breast and colon tumor pairs, respectively) compared to those produced by tissues that were snap-frozen. The correlation in the prediction of recurrence was 97% and 95% for the breast and colon tumor pairs, respectively, between these two types of tissue handling protocols. This novel finding demonstrates that prognostic signatures can be obtained from RNAlater preservative-suspended tissues, an important step in bringing gene expression signatures to the clinic.
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