ArticleMolecular biology of the cell2006
Heteromerization of innexin gap junction proteins regulates epithelial tissue organization in Drosophila.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
- Mechanisms Underlying the Initiation and Termination of Collective Cell Migration: Perspectives for Understanding Development and Cancer Metastasis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Dysregulation of Glial Gap Junction Protein Innexin2 Mediates Seizures and Disrupted Sleep in a Drosophila Model for North Sea Progressive Myoclonus Epilepsy.Journal of neuroscience research · 2025Article
- GABAergic neuronal dysfunction underlies tremor in spinocerebellar ataxia 3.Disease models & mechanisms · 2025Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae.PLoS genetics · 2024Article
- Maintenance of hematopoietic stem cell niche homeostasis requires gap junction-mediated calcium signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inbioRxiv : the preprint server for biology · 2023Article
- Bioelectric regulation of intestinal stem cells.Trends in cell biology · 2023Review
- Form and Function of the Vertebrate and Invertebrate Blood-Brain Barriers.International journal of molecular sciences · 2021Review
- Molecular and morphological approach to study the innexin gap junctions inOpen biology · 2021Article
- βPS-Integrin acts downstream of Innexin 2 in modulating stretched cell morphogenesis in the Drosophila ovary.G3 (Bethesda, Md.) · 2021Article
- Germline soma communication mediated by gap junction proteins regulates epithelial morphogenesis.PLoS genetics · 2021Article
- A Meta-Analysis of Bioelectric Data in Cancer, Embryogenesis, and Regeneration.Bioelectricity · 2021Article
- Article
- Integration of Migratory Cells into a New Site In Vivo Requires Channel-Independent Functions of Innexins on Microtubules.Developmental cell · 2020Article
- Gap Junction Channels of Innexins and Connexins: Relations and Computational Perspectives.International journal of molecular sciences · 2019Review
- Innexins: Expression, Regulation, and Functions.Frontiers in physiology · 2018Review
- Gap junctional signaling in pattern regulation: Physiological network connectivity instructs growth and form.Developmental neurobiology · 2017Review
- Gap junctions in C. elegans: Their roles in behavior and development.Developmental neurobiology · 2017Review
- Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis.BMC developmental biology · 2016Article
- Shaking B Mediates Synaptic Coupling between Auditory Sensory Neurons and the Giant Fiber of Drosophila melanogaster.PloS one · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gap junctions consist of clusters of intercellular channels, which enable direct cell-to-cell communication and adhesion in animals. Whereas deuterostomes, including all vertebrates, use members of the connexin and pannexin multiprotein families to assemble gap junction channels, protostomes such as Drosophila and Caenorhabditis elegans use members of the innexin protein family. The molecular composition of innexin-containing gap junctions and the functional significance of innexin oligomerization for development are largely unknown. Here, we report that heteromerization of Drosophila innexins 2 and 3 is crucial for epithelial organization and polarity of the embryonic epidermis. Both innexins colocalize in epithelial cell membranes. Innexin3 is mislocalized to the cytoplasm in innexin2 mutants and is recruited into ectopic expression domains defined by innexin2 misexpression. Conversely, RNA interference (RNAi) knockdown of innexin3 causes mislocalization of innexin2 and of DE-cadherin, causing cell polarity defects in the epidermis. Biochemical interaction studies, surface plasmon resonance analysis, transgenesis, and biochemical fractionation experiments demonstrate that both innexins interact via their C-terminal cytoplasmic domains during the assembly of heteromeric channels. Our data provide the first molecular and functional demonstration that innexin heteromerization occurs in vivo and reveal insight into a molecular mechanism by which innexins may oligomerize into heteromeric gap junction channels.
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