ArticleOncogene2006
The UVB-induced gene expression profile of human epidermis in vivo is different from that of cultured keratinocytes.
Article in Oncogene, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- Characterization of a human skin equivalent model to study the effects of ultraviolet B radiation on keratinocytes.Tissue engineering. Part C, Methods · 2014Trial
- In vivo identification of solar radiation-responsive gene network: role of the p38 stress-dependent kinase.PloS one · 2010Trial
- Proteomic analysis of the combined effects of cannabigerol and 3-O-ethyl ascorbic acid on kinase-dependent signalling in UVB-irradiated human keratinocytes.Scientific reports · 2024Article
- GLI Transcriptional Targets S100A7 and KRT16 Show Upregulated Expression Patterns in Epidermis Overlying the Tumor Mass in Melanoma Samples.International journal of molecular sciences · 2024Article
- Antioxidative and Skin Protective Effects ofEvidence-based complementary and alternative medicine : eCAM · 2021Article
- UVB Irradiation Induced Cell Damage and Early Onset ofAnimals : an open access journal from MDPI · 2020Article
- Dose and time effects of solar-simulated ultraviolet radiation on the in vivo human skin transcriptome.The British journal of dermatology · 2020Article
- Deciphering UV-induced DNA Damage Responses to Prevent and Treat Skin Cancer.Photochemistry and photobiology · 2020Review
- Molecular Subtyping and Prognostic Assessment Based on Tumor Mutation Burden in Patients with Lung Adenocarcinomas.International journal of molecular sciences · 2019Article
- AnFrontiers in microbiology · 2019Article
- Apigenin Inhibits UVB-Induced Skin Carcinogenesis: The Role of Thrombospondin-1 as an Anti-Inflammatory Factor.Neoplasia (New York, N.Y.) · 2018Article
- Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation.Scientific reports · 2017Article
- XRP44X, an Inhibitor of Ras/Erk Activation of the Transcription Factor Elk3, Inhibits Tumour Growth and Metastasis in Mice.PloS one · 2016Article
- Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging.The Journal of investigative dermatology · 2014Article
- Optimization and comprehensive characterization of a faithful tissue culture model of the benign and malignant human prostate.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Application of microRNA and mRNA expression profiling on prognostic biomarker discovery for hepatocellular carcinoma.BMC genomics · 2014Article
- Effects of narrow band UVB (311 nm) irradiation on epidermal cells.International journal of molecular sciences · 2013Review
- Regulators of global genome repair do not respond to DNA damaging therapy but correlate with survival in melanoma.PloS one · 2013Article
- SKINOMICS: Transcriptional Profiling in Dermatology and Skin Biology.Current genomics · 2012Article
- Human keratinocytes' response to injury upregulates CCL20 and other genes linking innate and adaptive immunity.The Journal of investigative dermatology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to obtain a comprehensive picture of the molecular events regulating cutaneous photodamage of intact human epidermis, suction blister roofs obtained after a single dose of in vivo ultraviolet (UV)B exposure were used for microarray profiling. We found a changed expression of 619 genes. Half of the UVB-regulated genes had returned to pre-exposure baseline levels at 72 h, underscoring the transient character of the molecular cutaneous UVB response. Of special interest was our finding that several of the central p53 target genes remained unaffected following UVB exposure in spite of p53 protein accumulation. We next compared the in vivo expression profiles of epidermal sheets to that of cultured human epidermal keratinocytes exposed to UVB in vitro. We found 1931 genes that differed in their expression profiles between the two groups. The expression profile in intact epidemis was geared mainly towards DNA repair, whereas cultured keratinocytes responded predominantly by activating genes associated with cell-cycle arrest and apoptosis. These differences in expression profiles might reflect differences between mature differentiating keratinocytes in the suprabasal epidermal layers versus exponentially proliferating keratinocytes in cell culture. Our findings show that extreme care should be taken when extrapolating from findings based on keratinocyte cultures to changes in intact epidermis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.