ArticleBMC infectious diseases2006
Synergistic effect of interleukin 1 alpha on nontypeable Haemophilus influenzae-induced up-regulation of human beta-defensin 2 in middle ear epithelial cells.
Article in BMC infectious diseases, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Subversion of host immune responses by otopathogens during otitis media.Journal of leukocyte biology · 2019Review
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- The Interplay Between Immune Response and Bacterial Infection in COPD: Focus Upon Non-typeableFrontiers in immunology · 2018Review
- Neuropeptides SP and CGRP Diminish theMediators of inflammation · 2018Article
- A proteomic characterization of NTHi lysates.International journal of pediatric otorhinolaryngology · 2016Article
- Haemophilus influenzae induces steroid-resistant inflammatory responses in COPD.BMC pulmonary medicine · 2015Article
- IL-10/HMOX1 signaling modulates cochlear inflammation via negative regulation of MCP-1/CCL2 expression in cochlear fibrocytes.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Intratympanic Gene Delivery of Antimicrobial Molecules in Otitis Media.Current allergy and asthma reports · 2015Review
- The Lung Immune Response to Nontypeable Haemophilus influenzae (Lung Immunity to NTHi).Journal of immunology research · 2015Review
- Botulinum neurotoxin type A induces TLR2-mediated inflammatory responses in macrophages.PloS one · 2015Article
- Role of innate immunity in the pathogenesis of otitis media.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2014Review
- Distal NF-kB binding motif functions as an enhancer for nontypeable H. influenzae-induced DEFB4 regulation in epithelial cells.Biochemical and biophysical research communications · 2014Article
- NOD2/RICK-dependent β-defensin 2 regulation is protective for nontypeable Haemophilus influenzae-induced middle ear infection.PloS one · 2014Article
- Mechanism of interleukin-1α transcriptional regulation of S100A9 in a human epidermal keratinocyte cell line.Biochimica et biophysica acta · 2013Article
- ERK2-dependent activation of c-Jun is required for nontypeable Haemophilus influenzae-induced CXCL2 upregulation in inner ear fibrocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Synergistic and feedback signaling mechanisms in the regulation of inflammation in respiratory infections.Cellular & molecular immunology · 2012Review
- Innate immunity and the role of defensins in otitis media.Current allergy and asthma reports · 2011Review
- Spiral ligament fibrocyte-derived MCP-1/CCL2 contributes to inner ear inflammation secondary to nontypeable H. influenzae-induced otitis media.BMC infectious diseases · 2010Article
- Mouse models for human otitis media.Brain research · 2009Review
- The expression of the beta-defensins hBD-2 and hBD-3 is differentially regulated by NF-kappaB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis.BMC immunology · 2009Article
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundWe recently showed that beta-defensins have antimicrobial activity against nontypeable Haemophilus influenzae (NTHi) and that interleukin 1 alpha (IL-1 alpha) up-regulates the transcription of beta-defensin 2 (DEFB4 according to new nomenclature of the Human Genome Organization) in human middle ear epithelial cells via a Src-dependent Raf-MEK1/2-ERK signaling pathway. Based on these observations, we investigated if human middle ear epithelial cells could release IL-1 alpha upon exposure to a lysate of NTHi and if this cytokine could have a synergistic effect on beta-defensin 2 up-regulation by the bacterial components.
methodsThe studies described herein were carried out using epithelial cell lines as well as a murine model of acute otitis media (OM). Human cytokine macroarray analysis was performed to detect the released cytokines in response to NTHi exposure. Real time quantitative PCR was done to compare the induction of IL-1 alpha or beta-defensin 2 mRNAs and to identify the signaling pathways involved. Direct activation of the beta-defensin 2 promoter was monitored using a beta-defensin 2 promoter-Luciferase construct. An IL-1 alpha blocking antibody was used to demonstrate the direct involvement of this cytokine on DEFB4 induction.
resultsMiddle ear epithelial cells released IL-1 alpha when stimulated by NTHi components and this cytokine acted in an autocrine/paracrine synergistic manner with NTHi to up-regulate beta-defensin 2. This synergistic effect of IL-1 alpha on NTHi-induced beta-defensin 2 up-regulation appeared to be mediated by the p38 MAP kinase pathway.
conclusionWe demonstrate that IL-1 alpha is secreted by middle ear epithelial cells upon exposure to NTHi components and that it can synergistically act with certain of these molecules to up-regulate beta-defensin 2 via the p38 MAP kinase pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.