ArticleExperimental brain research2006
Beta1-subunit modulates the Nav1.4 sodium channel by changing the surface charge.
Article in Experimental brain research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Sodium Channel β Subunits-An Additional Element in Animal Tetrodotoxin Resistance?International journal of molecular sciences · 2024Article
- Closed-state inactivation of cardiac, skeletal, and neuronal sodium channels is isoform specific.The Journal of general physiology · 2022Article
- Electrochemical modulation enhances the selectivity of peripheral neurostimulation in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The AMIGO1 adhesion protein activates Kv2.1 voltage sensors.Biophysical journal · 2022Article
- New Challenges Resulting From the Loss of Function of NaFrontiers in pharmacology · 2021Review
- Molecular Pathology of Sodium Channel Beta-Subunit Variants.Frontiers in pharmacology · 2021Article
- Chemometric Models of Differential Amino Acids at the NaMolecules (Basel, Switzerland) · 2020Article
- Effects of 4,9-anhydrotetrodotoxin on voltage-gated NaNaunyn-Schmiedeberg's archives of pharmacology · 2018Article
- Mutation E87Q of the β1-subunit impairs the maturation of the cardiac voltage-dependent sodium channel.Scientific reports · 2017Article
- Mechanisms of noncovalent β subunit regulation of NaThe Journal of general physiology · 2017Article
- On the multiple roles of the voltage gated sodium channel β1 subunit in genetic diseases.Frontiers in pharmacology · 2015Review
- A new look at sodium channel β subunits.Open biology · 2015Review
- Functional expression of Rat Nav1.6 voltage-gated sodium channels in HEK293 cells: modulation by the auxiliary β1 subunit.PloS one · 2014Article
- Functional modulation of voltage-dependent sodium channel expression by wild type and mutated C121W-β1 subunit.Journal of bioenergetics and biomembranes · 2013Article
- Molecular differential expression of voltage-gated sodium channel α and β subunit mRNAs in five different mammalian cell lines.Journal of bioenergetics and biomembranes · 2011Article
- Excitability constraints on voltage-gated sodium channels.PLoS computational biology · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
Voltage-gated sodium channels, comprised of a pore-forming alpha-subunit and additional regulatory (beta) subunits, play a critical role in regulation of neuronal excitability. Mechanisms of regulation of beta-subunits remain elusive. We have tested the functional effects of beta1 sodium channel subunit on surface charges as a mechanism for channel modulation. HEK-293 cell lines permanently transfected with the sole rat skeletal muscle sodium channel alpha-subunit (Nav1.4), or co-expressing the sodium channel alpha-subunit and beta1-subunit were studied with the whole-cell mode of the patch-clamp technique. At physiological extracellular Ca2+ concentration (2 mM), expression of beta1-subunit did not produce any significant effect on the voltage-dependent properties of sodium currents. However, a shift of half-activation potentials of sodium channel by changing the extracellular Ca2+ was potentiated when beta1 was co-expressed with alpha-subunit. In contrast, the expression of beta1-subunit did not affect the Ca2+ binding to the open or to the closed sodium channel pore, difference of the effect provoked by extracellular Ca2+ could therefore be attributed to an increased in negative surface charge determined by the presence of beta1-subunit. These data are in agreement with the hypothesis of a modulation of the sodium current by the expression of the highly sialylated beta1-subunit, which would alter the channel gating by increasing the density of surface negative charges in the vicinity of the sodium channel voltage sensing machinery.
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