ArticleBritish journal of pharmacology2006
Investigation of the role of TASK-2 channels in rat pulmonary arteries; pharmacological and functional studies following RNA interference procedures.
Article in British journal of pharmacology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- Pharmacological Modulation and (Patho)Physiological Roles of TRPM4 Channel-Part 1: Modulation of TRPM4.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Two-Pore-Domain Potassium (KCells · 2021Review
- Redox Regulation of Ion Channels and Receptors in Pulmonary Hypertension.Antioxidants & redox signaling · 2019Review
- Shared genetic risk between migraine and coronary artery disease: A genome-wide analysis of common variants.PloS one · 2017Article
- Hypoxia-dependent reactive oxygen species signaling in the pulmonary circulation: focus on ion channels.Antioxidants & redox signaling · 2015Review
- Regulation of breathing and autonomic outflows by chemoreceptors.Comprehensive Physiology · 2014Review
- Hypoxic pulmonary vasoconstriction.Physiological reviews · 2012Review
- Two-pore domain K⁺ channels regulate membrane potential of isolated human articular chondrocytes.The Journal of physiology · 2011Article
- Disruption of K(2P)6.1 produces vascular dysfunction and hypertension in mice.Hypertension (Dallas, Tex. : 1979) · 2011Article
- Contractile and electrophysiological properties of pulmonary artery smooth muscle are not altered in TASK-1 knockout mice.The Journal of physiology · 2011Article
- Cerebrovascular responses in mice deficient in the potassium channel, TREK-1.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Characterization of TWIK-2, a two-pore domain K+ channel, cloned from the rat middle cerebral artery.Experimental biology and medicine (Maywood, N.J.) · 2009Article
- Organ culture mimics the effects of hypoxia on membrane potential, K(+) channels and vessel tone in pulmonary artery.British journal of pharmacology · 2009Article
- cGMP does not activate two-pore domain K+ channels in cerebrovascular smooth muscle.American journal of physiology. Heart and circulatory physiology · 2009Article
- KCNQ modulators reveal a key role for KCNQ potassium channels in regulating the tone of rat pulmonary artery smooth muscle.The Journal of pharmacology and experimental therapeutics · 2009Article
- Two-pore potassium channels in the cardiovascular system.European biophysics journal : EBJ · 2009Review
- Mitochondria-dependent regulation of Kv currents in rat pulmonary artery smooth muscle cells.American journal of physiology. Lung cellular and molecular physiology · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we investigated the ability of RNA interference technology to suppress TASK-2 potassium channel expression in human embryonic kidney (HEK293) cells stably transfected with TASK-2 cDNA and in rat isolated intact pulmonary arteries. Lipofectamine-induced transfection of a specific siRNA sequence targeted against TASK-2 resulted in a dose- and time-dependent decrease in TASK-2 channel protein expression. In siRNA-transfected cells the TASK-2 peak currents were significantly smaller than in control cells at every investigated pH, while the pH sensitivity was not altered. Using scrambled siRNA as a negative control, there were no significant changes in TASK-2 protein expression or current compared to mock-transfected cells. In TASK-2 siRNA-transfected small pulmonary arteries, but not in scrambled siRNA-treated vessels, myocyte resting membrane potential at pH 7.4 was significantly less negative and the hyperpolarisations in response to increasing pH from 6.4 to 8.4 were significantly smaller compared with control. The application of levcromakalim (10 microM), NS1619 (33 microM) and a potassium channel inhibitor cocktail (5 mM 4-aminopyridine, 10 mM tetraethylammonium chloride, 30 microM Ba2+ and 10 microM glibenclamide) had similar effects in control and in siRNA-transfected vessels. The TASK-1 (anandamide-sensitive) contribution to resting membrane potential was comparable in each group. Clofilium (100 microM) generated significantly smaller responses in transfected artery segments. These results suggest that RNA interference techniques are effective at inhibiting TASK-2 channel expression in cultured cells and in intact vessels and that TASK-2 channels have a functional role in setting the membrane potential of pulmonary artery myocytes.
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