ArticleProceedings of the National Academy of Sciences of the United States of America2006
Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The impact of mitochondrial impairments on sperm function and male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2024Pooled it
- Improving fertilization rate in ICSI cycles by adding myoinositol to the semen preparation procedures: a prospective, bicentric, randomized trial on sibling oocytes.Journal of assisted reproduction and genetics · 2015Trial
- Paternal leakage of mitochondrial DNA is genetically controlled in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Monitoring the fate of paternal mitochondria and their elimination in rice zygotes.Plant physiology · 2026Article
- The nuclear and mitochondrial genomes need to tango. How is their dance synchronized during development?NAR molecular medicine · 2026Article
- Analysis of mtDNA Methylation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Phosphomimetic Experiments Do Not Support a Causal Role for TFAM Phosphorylation in mtDNA Elimination in Sperm.Journal of molecular biology · 2025Article
- Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights.Animals : an open access journal from MDPI · 2025Review
- Sperm mtDNA Copy Number Is Not Associated With Midpiece Size Among Songbirds.Ecology and evolution · 2025Article
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Endonuclease G is dispensable for sperm mitochondrial DNA elimination during spermatogenesis in mice.Biology open · 2024Article
- Egg multivesicular bodies elicit an LC3-associated phagocytosis-like pathway to degrade paternal mitochondria after fertilization.Nature communications · 2024Article
- Evidence for hybridization-driven heteroplasmy maintained across generations in a ricefish endemic to a Wallacean ancient lake.Biology letters · 2024Article
- ALLO-1- and IKKE-1-dependent positive feedback mechanism promotes the initiation of paternal mitochondrial autophagy.Nature communications · 2024Article
- Genome-wide methylation profile of mitochondrial DNA across bovine preimplantation development.Epigenetics · 2023Article
- Evolution, Inheritance, and Strata Formation of the W Chromosome in Duck (Anas platyrhynchos).Genome biology and evolution · 2023Article
- Effects of adverse fertility-related factors on mitochondrial DNA in the oocyte: a comprehensive review.Reproductive biology and endocrinology : RB&E · 2023Review
- Semi-in vitro detection of MgScientific reports · 2022Article
- Chirality and frequency measurement of longitudinal rolling of human sperm using optical trap.Frontiers in bioengineering and biotechnology · 2022Article
- Mitochondrial DNA in forensic use.Emerging topics in life sciences · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In almost all eukaryotes, mitochondrial (mt) genes are transmitted to progeny mainly from the maternal parent. The most popular explanation for this phenomenon is simple dilution of paternal mtDNA, because the paternal gametes (sperm) are much smaller than maternal gametes (egg) and contribute a limited amount of mitochondria to the progeny. Recently, this simple explanation has been challenged in several reports that describe the active digestion of sperm mtDNA, down-regulation of mtDNA replication in sperm, and proteolysis of mitochondria triggered by ubiquitination. In this investigation, we visualized mt nucleoids in living sperm by using highly sensitive SYBR green I vital staining. The ability to visualize mt nucleoids allowed us to clarify that the elimination of sperm mtDNA upon fertilization is achieved through two steps: (i) gradual decrease of mt nucleoid numbers during spermatogenesis and (ii) rapid digestion of sperm mtDNA just after fertilization. One notable point is that the digestion of mtDNA is achieved before the complete destruction of mitochondrial structures, which may be necessary to avoid the diffusion and transmission of potentially deleterious sperm mtDNA to the progeny.
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