ArticleProceedings of the National Academy of Sciences of the United States of America2006
In vivo identification of ribonucleoprotein-RNA interactions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Prospects and challenges in intracellular delivery of therapeutic proteins.Pharmacological reports : PR · 2026Review
- Structural Characterization of Protein-Nucleic Acid Complexes: An Overview of the Recent Innovation in the Analytic Methods.International journal of molecular sciences · 2025Review
- Decoding RNA-Protein Interactions: Methodological Advances and Emerging Challenges.Advanced genetics (Hoboken, N.J.) · 2025Review
- Towards an IdealInternational journal of molecular sciences · 2022Review
- Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection.Frontiers in cell and developmental biology · 2022Article
- Cell-Penetrating Peptides and Transportan.Pharmaceutics · 2021Review
- Compendium of Methods to Uncover RNA-Protein Interactions In Vivo.Methods and protocols · 2021Review
- Single and Combined Methods to Specifically or Bulk-Purify RNA-Protein Complexes.Biomolecules · 2020Review
- β-Actin mRNA interactome mapping by proximity biotinylation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Profiling the dress codes of RNA-binding proteins.Nature methods · 2018Article
- Article
- The Ever-Evolving Concept of the Gene: The Use of RNA/Protein Experimental Techniques to Understand Genome Functions.Frontiers in molecular biosciences · 2018Review
- Mass Spectrometry-Based Proteomics to Unveil the Non-coding RNA World.Frontiers in molecular biosciences · 2018Review
- Probing Long Non-coding RNA-Protein Interactions.Frontiers in molecular biosciences · 2017Review
- Effect of a Flexible Linker on Recombinant Expression of Cell-Penetrating Peptide Fusion Proteins and Their Translocation into Fungal Cells.Molecular biotechnology · 2016Article
- Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases.Nucleic acids research · 2016Review
- Novel RNA-Binding Proteins Isolation by the RaPID Methodology.Journal of visualized experiments : JoVE · 2016Article
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.International journal of molecular sciences · 2015Article
- High-throughput characterization of protein-RNA interactions.Briefings in functional genomics · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
To understand the role of RNA-binding proteins (RBPs) in the regulation of gene expression, methods are needed for the in vivo identification of RNA-protein interactions. We have developed the peptide nucleic acid (PNA)-assisted identification of RBP technology to enable the identification of proteins that complex with a target RNA in vivo. Specific regions of the 3' and 5' UTRs of ankylosis mRNA were targeted by antisense PNAs transported into cortical neurons by the cell-penetrating peptide transportan 10. An array of proteins was isolated in complex with or near the targeted regions of the ankylosis mRNA through UV-induced crosslinking of the annealed PNA-RNA-RBP complex. The first evidence for pharmacological modulation of these specific protein-RNA associations was observed. These data show that the PNA-assisted identification of the RBP technique is a reliable method to rapidly identify proteins interacting in vivo with the target RNA.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.