ArticleRNA (New York, N.Y.)2006
Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- An Arabidopsis pre-RNA processing8a (prp8a) missense allele restores splicing of a subset of mis-spliced mRNAs.Plant physiology · 2022Article
- The ChloroplastCells · 2021Review
- PRP8 Intein in Onygenales: Distribution and Phylogenetic Aspects.Mycopathologia · 2020Article
- Functional analysis of Cwc24 ZF-domain in 5' splice site selection.Nucleic acids research · 2019Article
- Dynamic protein-RNA interactions in mediating splicing catalysis.Nucleic acids research · 2019Article
- Structural dynamics of the N-terminal domain and the Switch loop of Prp8 during spliceosome assembly and activation.Nucleic acids research · 2018Review
- Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.Science (New York, N.Y.) · 2017Article
- Domestication of self-splicing introns during eukaryogenesis: the rise of the complex spliceosomal machinery.Biology direct · 2017Review
- Structural Insights into the Mechanism of Group II Intron Splicing.Trends in biochemical sciences · 2017Review
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
- Article
- CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.Current opinion in structural biology · 2016Review
- Article
- Spliceosome assembly in the absence of stable U4/U6 RNA pairing.RNA (New York, N.Y.) · 2015Article
- Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection.Nucleic acids research · 2015Article
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.Microbiology spectrum · 2015Review
- Group II intron lariat: Structural insights into the spliceosome.RNA biology · 2015Review
- Structural studies of the spliceosome: zooming into the heart of the machine.Current opinion in structural biology · 2014Review
- The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.RNA (New York, N.Y.) · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Current models of the core of the spliceosome include a network of RNA-RNA interactions involving the pre-mRNA and the U2, U5, and U6 snRNAs. The essential spliceosomal protein Prp8 interacts with U5 and U6 snRNAs and with specific pre-mRNA sequences that participate in catalysis. This close association with crucial RNA sequences, together with extensive genetic evidence, suggests that Prp8 could directly affect the function of the catalytic core, perhaps acting as a splicing cofactor. However, the sequence of Prp8 is almost entirely novel, and it offers few clues to the molecular basis of Prp8-RNA interactions. We have used an innovative transposon-based strategy to establish that catalytic core RNAs make multiple contacts in the central region of Prp8, underscoring the intimate relationship between this protein and the catalytic center of the spliceosome. Our analysis of RNA interactions identifies a discrete, highly conserved region of Prp8 as a prime candidate for the role of cofactor for the spliceosome's RNA core.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.