ArticleMolecular microbiology2006
Evidence that the SpoIIIE DNA translocase participates in membrane fusion during cytokinesis and engulfment.
Article in Molecular microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 62 citations in OpenAlex.
- A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis.Communications biology · 2026Article
- A single DNA methylation site regulates cell fate during Clostridioides difficile sporulation.PLoS pathogens · 2026Article
- Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation.Current biology : CB · 2022Article
- Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.Developmental cell · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- The molecular architecture of engulfment duringeLife · 2019Article
- Unraveling the Thousand Word Picture: An Introduction to Super-Resolution Data Analysis.Chemical reviews · 2017Article
- Development of Potent Antiviral Drugs Inspired by Viral Hexameric DNA-Packaging Motors with Revolving Mechanism.Journal of virology · 2016Review
- Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking.Nature biotechnology · 2016Article
- Missense Mutations Allow a Sequence-Blind Mutant of SpoIIIE to Successfully Translocate Chromosomes during Sporulation.PloS one · 2016Article
- Visualization and functional dissection of coaxial paired SpoIIIE channels across the sporulation septum.eLife · 2015Article
- Mechanistic study of classical translocation-dead SpoIIIE36 reveals the functional importance of the hinge within the SpoIIIE motor.Journal of bacteriology · 2014Article
- Structure and DNA-binding properties of the Bacillus subtilis SpoIIIE DNA translocase revealed by single-molecule and electron microscopies.Nucleic acids research · 2014Article
- Complete Genome of Bacillus thuringiensis Myophage BigBertha.Genome announcements · 2013Article
- The SpoIIQ landmark protein has different requirements for septal localization and immobilization.Molecular microbiology · 2013Article
- Visualization of pinholin lesions in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- FisB mediates membrane fission during sporulation in Bacillus subtilis.Genes & development · 2013Article
- Recruitment, assembly, and molecular architecture of the SpoIIIE DNA pump revealed by superresolution microscopy.PLoS biology · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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Abstract
During Bacillus subtilis sporulation, SpoIIIE is required for translocation of the trapped forespore chromosome across the sporulation septum, for compartmentalization of cell-specific gene expression, and for membrane fusion after engulfment. We isolated mutations within the SpoIIIE membrane domain that block localization and function. One mutant protein initially localizes normally and completes DNA translocation, but shows reduced membrane fusion after engulfment. Fluorescence recovery after photobleaching experiments demonstrate that in this mutant the sporulation septum remains open, allowing cytoplasmic contents to diffuse between daughter cells, suggesting that it blocks membrane fusion after cytokinesis as well as after engulfment. We propose that SpoIIIE catalyses these topologically opposite fusion events by assembling or disassembling a proteinaceous fusion pore. Mutants defective in SpoIIIE assembly also demonstrate that the ability of SpoIIIE to provide a diffusion barrier is directly proportional to its ability to assemble a focus at the septal midpoint during DNA translocation. Thus, SpoIIIE mediates compartmentalization by two distinct mechanisms: the SpoIIIE focus first provides a temporary diffusion barrier during DNA translocation, and then mediates the completion of membrane fusion after division to provide a permanent diffusion barrier. SpoIIIE-like proteins might therefore serve to couple the final step in cytokinesis, septal membrane fusion, to the completion of chromosome segregation.
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Registered trials
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