ArticlePlant molecular biology2006
Expression enhancement of a rice polyubiquitin gene promoter.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 78 citations in OpenAlex.
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Genome-Wide Identification and Characterization of UTR-Introns ofInternational journal of molecular sciences · 2020Article
- Expression characterization of the herbicide tolerance gene Aryloxyalkanoate Dioxygenase (aad-1) controlled by seven combinations of regulatory elements.BMC plant biology · 2018Article
- Article
- Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass.Plant biotechnology journal · 2016Article
- Characterization of the expression of the stress-responsivePlant biotechnology (Tokyo, Japan) · 2016Article
- BdCESA7, BdCESA8, and BdPMT Utility Promoter Constructs for Targeted Expression to Secondary Cell-Wall-Forming Cells of Grasses.Frontiers in plant science · 2016Article
- Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?Frontiers in plant science · 2016Review
- Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.Molecular biotechnology · 2015Article
- Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas.Planta · 2015Article
- The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.Plant cell reports · 2015Article
- A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Expression of hsp70, hsp100 and ubiquitin in Aloe barbadensis Miller under direct heat stress and under temperature acclimation conditions.Plant cell reports · 2013Article
- Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses.Plant molecular biology · 2013Article
- The UDP-glucuronate decarboxylase gene family in Populus: structure, expression, and association genetics.PloS one · 2013Article
- Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing.Nature biotechnology · 2012Article
- Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants.Plant cell reports · 2012Article
- Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation.BMC biotechnology · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An 808 bp promoter from a rice polyubiquitin gene, rubi3, has been isolated. The rubi3 gene contained an open reading frame of 1,140 bp encoding a pentameric polyubiquitin arranged as five tandem, head-to-tail repeats of 76 aa. The 1,140 bp 5' UTR intron of the gene enhanced its promoter activity in transient expression assays by 20-fold. Translational fusion of the GUS reporter gene to the coding sequence of the ubiquitin monomer enhanced GUS enzyme activity in transient expression assays by 4.3-fold over the construct containing the original rubi3 promoter (including the 5' UTR intron) construct. The enhancing effect residing in the ubiquitin monomer coding sequence has been narrowed down to the first 9 nt coding for the first three amino acid residues of the ubiquitin protein. Mutagenesis at the third nucleotide of this 9 nt sequence still maintains the enhancing effect, but leads to translation of the native GUS protein rather than a fusion protein. The resultant 5' regulatory sequence, consisting of the rubi3 promoter, 5' UTR exon and intron, and the mutated first 9 nt coding sequence, has an activity nearly 90-fold greater than the rubi3 promoter only (without the 5' UTR intron), and 2.2-fold greater than the maize Ubi1 gene promoter (including its 5' UTR intron). The newly created expression vector is expected to enhance transgene expression in monocot plants. Considering the high conservation of the polyubiquitin gene structure in higher plants, the observed enhancement in gene expression may apply to 5' regulatory sequences of other plant polyubiquitin genes.
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