ArticlePlant molecular biology2006
Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 107 citations in OpenAlex.
- Nano-enabled RNAi strategies for sustainable citrus protection against viral pathogens.Planta · 2026Review
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- Highly efficient hairy root genetic transformation and applications in citrus.Frontiers in plant science · 2022Article
- Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant.Plant cell reports · 2021Article
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- The unknown soldier in citrus plants: polyamines-based defensive mechanisms against biotic and abiotic stresses and their relationship with other stress-associated metabolites.Plant signaling & behavior · 2020Article
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
- Review
- Accumulation of 24 nucleotide transgene-derived siRNAs is associated with crinivirus immunity in transgenic plants.Molecular plant pathology · 2018Article
- The P25 protein of potato virus X (PVX) is the main pathogenicity determinant responsible for systemic necrosis in PVX-associated synergisms.Journal of virology · 2015Article
- Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection.Plant cell reports · 2013Article
- Citrus tristeza virus p23: a unique protein mediating key virus-host interactions.Frontiers in microbiology · 2013Article
- Citrus tristeza virus: Evolution of Complex and Varied Genotypic Groups.Frontiers in microbiology · 2013Article
- GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters.Plant cell reports · 2012Article
- Article
- Facilitative and antagonistic interactions between plant viruses in mixed infections.Molecular plant pathology · 2012Review
- Citrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile.Plant molecular biology · 2011Article
- Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance.Transgenic research · 2010Article
- Infection with strains of Citrus tristeza virus does not exclude superinfection by other strains of the virus.Journal of virology · 2010Article
- The pathogenicity determinant of Citrus tristeza virus causing the seedling yellows syndrome maps at the 3'-terminal region of the viral genome.Molecular plant pathology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific small interfering RNAs (siRNAs). When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. Other propagations were moderately resistant because they became infected later and showed attenuated symptoms compared to controls. The susceptible propagations, in addition to symptom expression and elevated virus titer, accumulated p23-specific siRNAs at levels significantly higher than immune or non-inoculated propagations, and showed transgene demethylation. This variable response among clonal transformants indicates that factors other than the genetic background of the transgenic plants play a key role in PTGS-mediated resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.