ArticleInfection and immunity2006
Expression microarray and mouse virulence analysis of four conserved two-component gene regulatory systems in group a streptococcus.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
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- Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group AInfection and immunity · 2020Article
- Polymorphisms in Regulator of Cov Contribute to the Molecular Pathogenesis of Serotype M28 Group A Streptococcus.The American journal of pathology · 2019Article
- In silico characterisation of the two-component system regulators of Streptococcus pyogenes.PloS one · 2018Article
- Increased Pilus Production Conferred by a Naturally Occurring Mutation Alters Host-Pathogen Interaction in Favor of Carriage in Streptococcus pyogenes.Infection and immunity · 2017Article
- Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III in Streptococcus pyogenes.Nucleic acids research · 2017Article
- YvqE and CovRS of Group A Streptococcus Play a Pivotal Role in Viability and Phenotypic Adaptations to Multiple Environmental Stresses.PloS one · 2017Article
- Zn2+ Uptake in Streptococcus pyogenes: Characterization of adcA and lmb Null Mutants.PloS one · 2016Article
- The FasX Small Regulatory RNA Negatively Regulates the Expression of Two Fibronectin-Binding Proteins in Group A Streptococcus.Journal of bacteriology · 2015Article
- A Single Amino Acid Replacement in the Sensor Kinase LiaS Contributes to a Carrier Phenotype in Group A Streptococcus.Infection and immunity · 2015Article
- Clinical laboratory response to a mock outbreak of invasive bacterial infections: a preparedness study.Journal of clinical microbiology · 2014Article
- Differential virulence gene expression of group A Streptococcus serotype M3 in response to co-culture with Moraxella catarrhalis.PloS one · 2013Article
- Inhibitor of streptokinase gene expression improves survival after group A streptococcus infection in mice.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Distinct single amino acid replacements in the control of virulence regulator protein differentially impact streptococcal pathogenesis.PLoS pathogens · 2011Article
- Serine/threonine protein kinase Stk is required for virulence, stress response, and penicillin tolerance in Streptococcus pyogenes.Infection and immunity · 2011Article
- SpyA, a C3-like ADP-ribosyltransferase, contributes to virulence in a mouse subcutaneous model of Streptococcus pyogenes infection.Infection and immunity · 2011Article
- Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins.BMC microbiology · 2011Article
- Adaptation of group A Streptococcus to human amniotic fluid.PloS one · 2010Article
- A decade of molecular pathogenomic analysis of group A Streptococcus.The Journal of clinical investigation · 2009Review
- CovS simultaneously activates and inhibits the CovR-mediated repression of distinct subsets of group A Streptococcus virulence factor-encoding genes.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group A streptococcus (GAS) is a gram-positive human bacterial pathogen that causes diseases ranging from relatively mild epithelial cell surface infections to life-threatening invasive episodes. Much is known about the extracellular molecules that contribute to host-pathogen interactions, but in contrast, far less information is available about regulatory genes that control the expression of individual or multiple GAS virulence factors. The eight GAS genomes that have been sequenced have 12 conserved two-component gene regulatory systems (TCSs), but only 3 of these 12 have been studied in detail. Using an allelic replacement strategy with a nonpolar cassette, we inactivated the response regulator of four TCSs that have only weak homology with TCS genes of known or inferred function in other bacteria. The mutant strains were analyzed by expression microarray analysis at four time points and tested in two mouse infection models. Each TCS influenced expression (directly or indirectly) of 12 to 41% of all chromosomal genes, as assessed by growth in Todd-Hewitt broth and a custom Affymetrix GeneChip. None of the isogenic mutant strains was significantly altered for mouse virulence based on intraperitoneal inoculation. Similarly, compared to the wild-type strain, there was no significant difference in skin lesion size for three of the four mutants. In contrast, the DeltaM5005_Spy_0680 mutant strain produced significantly larger abscesses after subcutaneous inoculation into mice, consistent with a hypervirulence phenotype. The mutant strain had significantly higher in vitro expression of several proven and putative virulence genes, including scpA, encoding a peptidase that inactivates complement protein C5a. Together, the data provide new information about previously uncharacterized GAS TCSs.
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