ArticleInfection and immunity2006
The established intimin receptor Tir and the putative eucaryotic intimin receptors nucleolin and beta1 integrin localize at or near the site of enterohemorrhagic Escherichia coli O157:H7 adherence to enterocytes in vivo.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Both pathogen and host dynamically adapt pH responses along the intestinal tract during enteric bacterial infection.PLoS biology · 2024Article
- ThemSphere · 2024Article
- Nucleolin: a cell portal for viruses, bacteria, and toxins.Cellular and molecular life sciences : CMLS · 2022Review
- Serotype-dependent adhesion of Shiga toxin-producingFrontiers in microbiology · 2022Article
- Differential expression and correlation analysis of miRNA-mRNA profiles in swine testicular cells infected with porcine epidemic diarrhea virus.Scientific reports · 2021Article
- Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7.BMC genomics · 2019Article
- Article
- Enterohaemorrhagic Escherichia coli inhibits recycling endosome function and trafficking of surface receptors.Cellular microbiology · 2014Article
- Analysis of the virulence of an atypical enteropathogenic Escherichia coli strain in vitro and in vivo and the influence of type three secretion system.BioMed research international · 2014Article
- Growth media simulating ileal and colonic environments affect the intracellular proteome and carbon fluxes of enterohemorrhagic Escherichia coli O157:H7 strain EDL933.Applied and environmental microbiology · 2013Article
- RSV fusion: time for a new model.Viruses · 2013Review
- Molecular mechanisms that mediate colonization of Shiga toxin-producing Escherichia coli strains.Infection and immunity · 2012Review
- Protection against Escherichia coli O157:H7 challenge by immunization of mice with purified Tir proteins.Molecular biology reports · 2012Article
- Cell-surface nucleolin is sequestered into EPEC microcolonies and may play a role during infection.Microbiology (Reading, England) · 2011Article
- Identification and characterisation of a novel adhesin Ifp in Yersinia pseudotuberculosis.BMC microbiology · 2011Article
- A new twist on plasma membrane repair.Communicative & integrative biology · 2011Article
- Mouse models of Escherichia coli O157:H7 infection and shiga toxin injection.Journal of biomedicine & biotechnology · 2011Review
- Article
- Enterohemorrhagic E. coli alters murine intestinal epithelial tight junction protein expression and barrier function in a Shiga toxin independent manner.Laboratory investigation; a journal of technical methods and pathology · 2010Article
- Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coli O157:H7 in cattle.BMC veterinary research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
For enterohemorrhagic Escherichia coli (EHEC) O157:H7 to adhere tightly to the intestinal epithelium and produce attach and efface (A/E) lesions, the organism must express the adhesin intimin and insert the bacterially encoded translocated intimin receptor Tir into the plasma membrane of the host enterocyte. Additionally, some reports based on tissue culture experiments indicate that intimin has affinity for the eucaryotic proteins nucleolin and beta1 integrin. To address the potential biological relevance of these eucaryotic proteins in the infection process in vivo, we sought to compare the proximity of Tir, nucleolin, and beta1 integrin to regions of EHEC O157:H7 attachment in intestinal sections from three different inoculated animals: piglets, neonatal calves, and mice. Piglets and neonatal calves were chosen because intimin-mediated adherence of EHEC O157:H7 and subsequent A/E lesion formation occur at high levels in these animals. Mice were selected because of their ease of manipulation but only after we first demonstrated that in competition with the normal mouse gut flora, an EHEC O157:H7 strain with a nonpolar deletion in the intimin gene was cleared faster than strains that produced wild-type or hybrid intimin. In all three animal species, we noted immunostained Tir beneath and stained nucleolin closely associated with adherent bacteria in intestinal sections. We also observed immunostained beta1 integrin clustered at locations of bacterial adherence in porcine and bovine tissue. These findings indicate that nucleolin and beta1 integrin are present on the luminal surface of intestinal epithelia and are potentially accessible as receptors for intimin during EHEC O157:H7 infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.