ArticleInfection and immunity2006
Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Innovation and Application of the Type III Secretion System Inhibitors in Plant Pathogenic Bacteria.Microorganisms · 2020Review
- Review
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- Discovering RNA-Based Regulatory Systems forFrontiers in cellular and infection microbiology · 2018Review
- RNA-based mechanisms of virulence control in Enterobacteriaceae.RNA biology · 2017Review
- Neisseria meningitidis Polynucleotide Phosphorylase Affects Aggregation, Adhesion, and Virulence.Infection and immunity · 2016Article
- The Typhoid Toxin Promotes Host Survival and the Establishment of a Persistent Asymptomatic Infection.PLoS pathogens · 2016Article
- Exposure of Salmonella enterica Serovar Typhimurium to Three Humectants Used in the Food Industry Induces Different Osmoadaptation Systems.Applied and environmental microbiology · 2015Article
- Defects in polynucleotide phosphorylase impairs virulence in Escherichia coli O157:H7.Frontiers in microbiology · 2015Article
- HSPC117 is regulated by epigenetic modification and is involved in the migration of JEG-3 cells.International journal of molecular sciences · 2014Article
- Comparative analysis of Salmonella susceptibility and tolerance to the biocide chlorhexidine identifies a complex cellular defense network.Frontiers in microbiology · 2014Article
- The Aeromonas salmonicida subsp. salmonicida exoproteome: determination of the complete repertoire of Type-Three Secretion System effectors and identification of other virulence factors.Proteome science · 2013Article
- ProP is required for the survival of desiccated Salmonella enterica serovar typhimurium cells on a stainless steel surface.Applied and environmental microbiology · 2013Article
- The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens.Frontiers in cellular and infection microbiology · 2013Review
- Crystallization and preliminary X-ray diffraction studies of Xanthomonas campestris PNPase in the presence of c-di-GMP.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly.Open biology · 2012Article
- Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni.Frontiers in cellular and infection microbiology · 2012Article
- Ribonucleases and bacterial virulence.Microbial biotechnology · 2011Review
- Polynucleotide phosphorylase activity may be modulated by metabolites in Escherichia coli.The Journal of biological chemistry · 2011Article
- Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Mutational inactivation of the cold-shock-associated exoribonuclease polynucleotide phosphorylase (PNPase; encoded by the pnp gene) in Salmonella enterica serovar Typhimurium was previously shown to enable the bacteria to cause chronic infection and to affect the bacterial replication in BALB/c mice (M. O. Clements et al., Proc. Natl. Acad. Sci. USA 99:8784-8789, 2002). Here, we report that PNPase deficiency results in increased expression of Salmonella plasmid virulence (spv) genes under in vitro growth conditions that allow induction of spv expression. Furthermore, whole-genome microarray-based transcriptome analyses of bacteria growing inside murine macrophage-like J774.A.1 cells revealed six genes as being significantly up-regulated in the PNPase-deficient background, which included spvABC, rtcB, entC, and STM2236. Mutational inactivation of the spvR regulator diminished the increased expression of spv observed in the pnp mutant background, implying that PNPase acts upstream of or at the level of SpvR. Finally, competition experiments revealed that the growth advantage of the pnp mutant in BALB/c mice was dependent on spvR as well. Combined, our results support the idea that in S. enterica PNPase, apart from being a regulator of the cold shock response, also functions in tuning the expression of virulence genes and bacterial fitness during infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.