ArticleInfection and immunity2006
HpaA is essential for Helicobacter pylori colonization in mice.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 86 citations in OpenAlex.
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- An M cell-targeting recombinant L. lactis vaccine against four H. pylori adhesins.Applied microbiology and biotechnology · 2024Article
- Modeling and optimization of culture media for recombinantFrontiers in bioengineering and biotechnology · 2024Article
- Prediction of gastric cancer risk by a polygenic risk score ofWorld journal of gastrointestinal oncology · 2022Article
- Transcriptional and functional characterizations of multiple flagellin genes in spirochetes.Molecular microbiology · 2022Article
- Protective immunity enhancedFrontiers in immunology · 2022Article
- Hesperetin Inhibits Expression of Virulence Factors and Growth ofInternational journal of molecular sciences · 2021Article
- Sialic Acids as Receptors for Pathogens.Biomolecules · 2021Review
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- A Novel Multi-Epitopic Peptide Vaccine Candidate AgainstInternational journal of peptide research and therapeutics · 2021Article
- Phylogenetic Distribution, Ultrastructure, and Function of Bacterial Flagellar Sheaths.Biomolecules · 2020Review
- Identification of Functional Interactome of Gastric Cancer Cells withBioMed research international · 2020Article
- Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori.Journal of nanobiotechnology · 2019Article
- Synthetic Lipopeptide Enhances Protective Immunity AgainstFrontiers in immunology · 2019Article
- A GWAS on Helicobacter pylori strains points to genetic variants associated with gastric cancer risk.BMC biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infection with the human gastric pathogen Helicobacter pylori can give rise to chronic gastritis, peptic ulcer, and gastric cancer. All H. pylori strains express the surface-localized protein HpaA, a promising candidate for a vaccine against H. pylori infection. To study the physiological importance of HpaA, a mutation of the hpaA gene was introduced into a mouse-adapted H. pylori strain. To justify that the interruption of the hpaA gene did not cause any polar effects of downstream genes or was associated with a second site mutation, the protein expression patterns of the mutant and wild-type strains were characterized by two different proteomic approaches. Two-dimensional differential in-gel electrophoresis analysis of whole-cell extracts and subcellular fractionation combined with nano-liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry for outer membrane protein profiling revealed only minor differences in the protein profile between the mutant and the wild-type strains. Therefore, the mutant strain was tested for its colonizing ability in a well-established mouse model. While inoculation with the wild-type strain resulted in heavily H. pylori-infected mice, the HpaA mutant strain was not able to establish colonization. Thus, by combining proteomic analysis and in vivo studies, we conclude that HpaA is essential for the colonization of H. pylori in mice.
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