ArticleInfection and immunity2006
Analysis of the function of enteropathogenic Escherichia coli EspB by random mutagenesis.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- The sequence of events of enteropathogeniciScience · 2024Article
- Bioprospecting of inhibitors of EPEC virulence from metabolites of marine actinobacteria from the Arctic Sea.Frontiers in microbiology · 2024Article
- Assay for Type III Secretion in Escherichia coli.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenicVirulence · 2021Article
- AtaT Improves the Stability of Pore-Forming Protein EspB by Acetylating Lysine 206 to Enhance Strain Virulence.Frontiers in microbiology · 2021Article
- Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens.International journal of molecular sciences · 2020Review
- Article
- Epithelial cells detect functional type III secretion system of enteropathogenic Escherichia coli through a novel NF-κB signaling pathway.PLoS pathogens · 2017Article
- Inhibition of Adhesion of Enteropathogenic Escherichia coli to HEp-2 Cells by Binding of a Novel Peptide to EspB Protein.Current microbiology · 2016Article
- Bacterial Control of Pores Induced by the Type III Secretion System: Mind the Gap.Frontiers in immunology · 2016Review
- SepD/SepL-dependent secretion signals of the type III secretion system translocator proteins in enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Enteropathogenic escherichia coli infection in children.Current opinion in infectious diseases · 2011Review
- The N-terminal amphipathic region of the Escherichia coli type III secretion system protein EspD is required for membrane insertion and function.Molecular microbiology · 2011Article
- Interactions and predicted host membrane topology of the enteropathogenic Escherichia coli translocator protein EspB.Journal of bacteriology · 2011Article
- A bacterial effector targets host DH-PH domain RhoGEFs and antagonizes macrophage phagocytosis.The EMBO journal · 2010Article
- Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli.Molecular microbiology · 2009Article
- Rapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espD.Biological procedures online · 2007Article
- Pathophysiology of EnteropathogenicNewborn (Clarksville, Md.)Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enteropathogenic Escherichia coli (EPEC) is an important cause of infantile diarrhea, especially in developing countries. EspB, a key virulence factor of EPEC, is required for the attaching and effacing effect characteristic of EPEC and enterohemorrhagic E. coli and has been posited to play several functions in the process of infection. Attaching and effacing activity is associated with the accumulation of filamentous actin beneath adherent bacteria as measured in the fluorescence actin staining (FAS) test. To determine whether different domains of EspB are responsible for different functions, 42 plasmids carrying mutated espB were introduced into an espB deletion mutant. Two major groups of espB mutants were identified. One group of 17 mutants exhibited positive FAS results and normal levels of hemolytic activity. Another group of 22 mutants exhibited negative FAS results and low levels of hemolytic activity. Three mutants were exceptional. One mutant was FAS positive but had significantly reduced hemolytic activity. Conversely, a second mutant was FAS negative but had full hemolytic activity. A third mutant had a significantly reduced FAS level compared to the wild type but full hemolytic activity. The results of EspF and Tir translocation assays confirmed that FAS-negative insertions disrupt effector translocation and mutants with FAS-positive insertions retain protein translocation activity. These results suggest that EspB has distinct domain functions involved in effector translocation that can be distinguished from its role as a component of the translocation pore.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.