ArticleMolecular and cellular biology2006
Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 169 citations in OpenAlex.
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- A chimeric rather than native secretion signal elicits stress-related transcriptional adaption during 75k γ-secalin production inFEMS microbes · 2026Article
- Retrofitting of AOX1 Promoter-Based Pichia pastoris Expression Strains for Methanol-Independent Protein Production.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Enhancing recombinant thaumatin production by Komagataella phaffii through cold atmospheric helium plasma jet.Scientific reports · 2025Article
- Yeast Midas' touch: recent advances in the valorisation of methanol by yeasts.World journal of microbiology & biotechnology · 2025Review
- ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13.The Journal of cell biology · 2025Article
- Evaluation of the immune effect of foot-and-mouth disease virus-like particles derived fromFrontiers in microbiology · 2025Article
- Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators inSynthetic and systems biotechnology · 2025Article
- Recent progress on heterologous protein production in methylotrophic yeast systems.World journal of microbiology & biotechnology · 2024Review
- Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii.Microbial cell factories · 2024Article
- Unlocking Nature's Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter.Microbial cell factories · 2024Article
- Yeast transcriptional device libraries enable precise synthesis of value-added chemicals from methanol.Nucleic acids research · 2022Article
- Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeastProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Histidine is essential for growth of Komagataella phaffii cultured in YPA medium.FEBS open bio · 2022Article
- Article
- A programmable high-expression yeast platform responsive to user-defined signals.Science advances · 2022Article
- Coordinate regulation of methanol utilization pathway genes ofFrontiers in microbiology · 2022Article
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic YeastFrontiers in cell and developmental biology · 2022Review
- Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the YeastMicroorganisms · 2021Article
12 more citing papers are in PubMed but not listed here.
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11 authors at 4 institutions in 2 countries.
Funding
Abstract
Growth of the yeast Pichia pastoris on methanol induces the expression of genes whose products are required for its metabolism. Three of the methanol pathway enzymes are located in an organelle called the peroxisome. As a result, both methanol pathway enzymes and proteins involved in peroxisome biogenesis (PEX proteins) are induced in response to this substrate. The most highly regulated of these genes is AOX1, which encodes alcohol oxidase, the first enzyme of the methanol pathway, and a peroxisomal enzyme. To elucidate the molecular mechanisms responsible for methanol regulation, we identify genes required for the expression of AOX1. Mutations in one gene, named MXR1 (methanol expression regulator 1), result in strains that are unable to (i) grow on the peroxisomal substrates methanol and oleic acid, (ii) induce the transcription of AOX1 and other methanol pathway and PEX genes, and (iii) form normal-appearing peroxisomes in response to methanol. MXR1 encodes a large protein with a zinc finger DNA-binding domain near its N terminus that has similarity to Saccharomyces cerevisiae Adr1p. In addition, Mxr1p is localized to the nucleus in cells grown on methanol or other gluconeogenic substrates. Finally, Mxr1p specifically binds to sequences upstream of AOX1. We conclude that Mxr1p is a transcription factor that is necessary for the activation of many genes in response to methanol. We propose that MXR1 is the P. pastoris homologue of S. cerevisiae ADR1 but that it has gained new functions and lost others through evolution as a result of changes in the spectrum of genes that it controls.
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