ArticleMolecular and cellular biology2006
Real-time monitoring of chloroplast gene expression by a luciferase reporter: evidence for nuclear regulation of chloroplast circadian period.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas.Nature plants · 2025Article
- Diurnal-Rhythmic Relationships between Physiological Parameters and Photosynthesis- and Antioxidant-Enzyme Genes Expression in the RaphidophyteAntioxidants (Basel, Switzerland) · 2024Article
- Accelerating Chloroplast Engineering: A New System for Rapid Generation of Marker-Free Transplastomic Lines ofMicroorganisms · 2023Article
- Mechanisms governing codon usage bias and the implications for protein expression in the chloroplast of Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2022Review
- The circadian night depression of photosynthesis analyzed in a herb, Pulmonaria vallarsae. Day/night quantitative relationships.Photosynthesis research · 2022Article
- A potential EARLY FLOWERING 3 homolog in Chlamydomonas is involved in the red/violet and blue light signaling pathways for the degradation of RHYTHM OF CHLOROPLAST 15.PLoS genetics · 2022Article
- Evolution of circadian clocks along the green lineage.Plant physiology · 2022Article
- Responses of butter leaf lettuce to mixed red and blue light with extended light/dark cycle period.Scientific reports · 2022Article
- Nanotechnology Approaches for Chloroplast Biotechnology Advancements.Frontiers in plant science · 2021Review
- The role of ROC75 as a daytime component of the circadian oscillator in Chlamydomonas reinhardtii.PLoS genetics · 2020Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Review
- CSL encodes a leucine-rich-repeat protein implicated in red/violet light signaling to the circadian clock in Chlamydomonas.PLoS genetics · 2017Article
- A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Diversity of plant circadian clocks: Insights from studies of Chlamydomonas reinhardtii and Physcomitrella patens.Plant signaling & behavior · 2016Article
- Nondestructive and intuitive determination of circadian chlorophyll rhythms in soybean leaves using multispectral imaging.Scientific reports · 2015Article
- Chlamydomonas reinhardtii strain CC-124 is highly sensitive to blue light in addition to green and red light in resetting its circadian clock, with the blue-light photoreceptor plant cryptochrome likely acting as negative modulator.Plant physiology and biochemistry : PPB · 2014Article
- The circadian regulation of photosynthesis.Photosynthesis research · 2014Review
- The diurnal logic of the expression of the chloroplast genome in Chlamydomonas reinhardtii.PloS one · 2014Article
- Codon-optimized antibiotic resistance gene improves efficiency of transient transformation in Frankia.Journal of biosciences · 2013Article
Corrections and comments
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5 authors.
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Abstract
Chloroplast-encoded genes, like nucleus-encoded genes, exhibit circadian expression. How the circadian clock exerts its control over chloroplast gene expression, however, is poorly understood. To facilitate the study of chloroplast circadian gene expression, we developed a codon-optimized firefly luciferase gene for the chloroplast of Chlamydomonas reinhardtii as a real-time bioluminescence reporter and introduced it into the chloroplast genome. The bioluminescence of the reporter strain correlated well with the circadian expression pattern of the introduced gene and satisfied all three criteria for circadian rhythms. Moreover, the period of the rhythm was lengthened in per mutants, which are phototactic rhythm mutants carrying a long-period gene in their nuclear genome. These results demonstrate that chloroplast gene expression rhythm is a bona fide circadian rhythm and that the nucleus-encoded circadian oscillator determines the period length of the chloroplast rhythm. Our reporter strains can serve as a powerful tool not only for analysis of the circadian regulation mechanisms of chloroplast gene expression but also for a genetic approach to the molecular oscillator of the algal circadian clock.
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