ArticleJournal of bacteriology2006
Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium meliloti.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 120 citations in OpenAlex.
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- Three plant growth-promoting rhizobacteria from the Qinghai-Tibet Plateau enhanced plant growth and alleviated low-temperature and drought stress.Frontiers in plant science · 2026Article
- Polyhydroxyalkanoate synthesis by Sinorhizobium meliloti drives a host-specific collapse in symbiosis with Medicago sativa.Research square · 2025Article
- Effects of different land use on functional genes of soil microbial carbon and phosphorus cycles in the desert steppe zone of the Loess Plateau.BMC microbiology · 2025Article
- Unexpected modulation of Hna phage defense activity by the symbiotic regulator NolR.Journal of bacteriology · 2025Article
- Biodegradation of glyphosate by Stenotrophomonas maltophilia GP-1 involves C-P lyase pathway.Biodegradation · 2025Article
- A minimized symbiotic gene set from the 1.68 Mb pSymB chromid of Sinorhizobium meliloti reveals auxiliary symbiotic loci.BMC biology · 2025Article
- Adaptations of the GenusCurrent issues in molecular biology · 2025Review
- Landscape of the metaplasmidome of deep-sea hydrothermal vents located at Arctic Mid-Ocean Ridges in the Norwegian-Greenland Sea: ecological insights from comparative analysis of plasmid identification tools.FEMS microbiology ecology · 2024Article
- Identification and functional analysis of recent IS transposition events in rhizobia.Mobile DNA · 2024Article
- Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection.Environmental science and ecotechnology · 2024Review
- Analysis of CenKR essentiality in Sinorhizobium meliloti and its activity at a target gene promoter in vivo.FEMS microbiology letters · 2024Article
- Effect of ammonium stress on phosphorus solubilization of a novel marine mangrove microorganism Bacillus aryabhattai NM1-A2 as revealed by integrated omics analysis.BMC genomics · 2023Article
- The transport of mannitol inMicrobiology (Reading, England) · 2023Article
- Review
- The two-component regulatory system CenK-CenR regulates expression of a previously uncharacterized protein required for salinity and oxidative stress tolerance inFrontiers in microbiology · 2022Article
- TheFrontiers in microbiology · 2022Article
- Transcriptomics differentiate two novel bioactive strains of Paenibacillus sp. isolated from the perennial ryegrass seed microbiome.Scientific reports · 2021Article
- Differentially expressed microRNAs that target functional genes in mature soybean nodules.The plant genome · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The properties and regulation of the pstSCAB-encoded Pi uptake system from the alfalfa symbiont Sinorhizobium meliloti are reported. We present evidence that the pstSCAB genes and the regulatory phoUB genes are transcribed from a single promoter that contains two PhoB binding sites and that transcription requires PhoB. S. meliloti strain 1021 (Rm1021) and its derivatives were found to carry a C deletion frameshift mutation in the pstC gene (designated pstC1021) that severely impairs activity of the PstSCAB Pi transport system. This mutation is absent in RCR2011, the parent of Rm1021. Correction of the pstC1021 mutation in Rm1021 by site-directed mutagenesis revealed that PstSCAB is a Pi-specific, high-affinity (Km, 0.2 microM), high-velocity (Vmax, 70 nmol/min/mg protein) transport system. The pstC1021 allele was shown to generate a partial pho regulon constitutive phenotype, in which transcription is activated by PhoB even under Pi-excess conditions that render PhoB inactive in a wild-type background. The previously reported symbiotic Fix- phenotype of phoCDET mutants was found to be dependent on the pstC1021 mutation, as Rm1021 phoCDET mutants formed small white nodules on alfalfa that failed to reduce N2, whereas phoCDET mutant strains with a corrected pstC allele (RmP110) formed pink nodules on alfalfa that fixed N2 like the wild type. Alfalfa root nodules formed by the wild-type RCR2011 strain expressed the low-affinity orfA-pit-encoded Pi uptake system and neither the pstSCAB genes nor the phoCDET genes. Thus, metabolism of alfalfa nodule bacteroids is not Pi limited.
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