ArticleJournal of bacteriology2006
Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Uncovering transcriptional processes in microbial communities adapted to differing saline conditions in salt-weathered historic buildings.Microbiome · 2026Article
- Stochastic Character Mapping, Bayesian Model Selection, and Biosynthetic Pathways Shed New Light on the Evolution of Habitat Preference in Cyanobacteria.Systematic biology · 2024Article
- Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12.Biochemistry · 2018Article
- Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism.Applied and environmental microbiology · 2017Article
- Mycobacterium hassiacum recovers from nitrogen starvation with up-regulation of a novel glucosylglycerate hydrolase and depletion of the accumulated glucosylglycerate.Scientific reports · 2014Article
- Mannosylglycerate: structural analysis of biosynthesis and evolutionary history.Extremophiles : life under extreme conditions · 2014Review
- Mechanism of dephosphorylation of glucosyl-3-phosphoglycerate by a histidine phosphatase.The Journal of biological chemistry · 2014Article
- Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica.Scientific reports · 2013Article
- Gluconeotrehalose is the principal organic solute in the psychrotolerant bacterium Carnobacterium strain 17-4.Extremophiles : life under extreme conditions · 2011Article
- Mycobacterium tuberculosis Rv2419c, the missing glucosyl-3-phosphoglycerate phosphatase for the second step in methylglucose lipopolysaccharide biosynthesis.Scientific reports · 2011Article
- Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis.Journal of bacteriology · 2010Article
- Crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Article
- Single-step pathway for synthesis of glucosylglycerate in Persephonella marina.Journal of bacteriology · 2007Article
- Glucosylglycerate biosynthesis in the deepest lineage of the Bacteria: characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina.Journal of bacteriology · 2007Article
- Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis.Folia microbiologica · 2007Article
- Biosynthetic pathways of inositol and glycerol phosphodiesters used by the hyperthermophile Archaeoglobus fulgidus in stress adaptation.Journal of bacteriology · 2006Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathway for the synthesis of the organic solute glucosylglycerate (GG) is proposed based on the activities of the recombinant glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) from Methanococcoides burtonii. A mannosyl-3-phosphoglycerate phosphatase gene homologue (mpgP) was found in the genome of M. burtonii (http://www.jgi.doe.gov), but an mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) was absent. The gene upstream of the mpgP homologue encoded a putative glucosyltransferase that was expressed in Escherichia coli. The recombinant product had GpgS activity, catalyzing the synthesis of glucosyl-3-phosphoglycerate (GPG) from GDP-glucose and d-3-phosphoglycerate, with a high substrate specificity. The recombinant MpgP protein dephosphorylated GPG to GG and was also able to dephosphorylate mannosyl-3-phosphoglycerate (MPG) but no other substrate tested. Similar flexibilities in substrate specificity were confirmed in vitro for the MpgPs from Thermus thermophilus, Pyrococcus horikoshii, and "Dehalococcoides ethenogenes." GpgS had maximal activity at 50 degrees C. The maximal activity of GpgP was at 50 degrees C with GPG as the substrate and at 60 degrees C with MPG. Despite the similarity of the sugar donors GDP-glucose and GDP-mannose, the enzymes for the synthesis of GPG or MPG share no amino acid sequence identity, save for short motifs. However, the hydrolysis of GPG and MPG is carried out by phosphatases encoded by homologous genes and capable of using both substrates. To our knowledge, this is the first report of the elucidation of a biosynthetic pathway for glucosylglycerate.
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