ArticleJournal of bacteriology2006
Anatomy of the replication origin of plasmid ColE2-P9.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 28 citations in OpenAlex.
- Structure and functions of a multireplicon genome of Antarctic Psychrobacter sp. ANT_H3: characterization of the genetic modules suitable for the construction of the plasmid-vectors for cold-active bacteria.Journal of applied genetics · 2023Article
- Synthetic bacteria for the detection and bioremediation of heavy metals.Frontiers in bioengineering and biotechnology · 2023Review
- Genomic and proteomic characterization of two strains of Shigella flexneri 2 isolated from infants' stool samples in Argentina.BMC genomics · 2022Article
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- Next-generation biocontainment systems for engineered organisms.Nature chemical biology · 2018Article
- Plasmid Replicons fromFrontiers in microbiology · 2017Article
- Structural basis for replication origin unwinding by an initiator primase of plasmid ColE2-P9: duplex DNA unwinding by a single protein.The Journal of biological chemistry · 2015Article
- Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.Plasmid · 2015Article
- Plasmid diversity in arctic strains of Psychrobacter spp.Extremophiles : life under extreme conditions · 2013Article
- Evolutionary link between the mycobacterial plasmid pAL5000 replication protein RepB and the extracytoplasmic function family of σ factors.Journal of bacteriology · 2012Article
- DNA bending in the mycobacterial plasmid pAL5000 origin-RepB complex.Journal of bacteriology · 2007Article
- Distinct functions of the two specificity determinants in replication initiation of plasmids ColE2-P9 and ColE3-CA38.Journal of bacteriology · 2007Article
- Specific interaction between the initiator protein (Rep) and origin of plasmid ColE2-P9.Journal of bacteriology · 2007Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plasmid ColE2-P9 origin is a 32-bp region which is specifically recognized by the plasmid-specified Rep protein to initiate DNA replication. We analyzed the structural and functional organization of the ColE2 origin by using various derivatives carrying deletions and single-base-pair substitutions. The origin may be divided into three subregions: subregion I, which is important for stable binding of the Rep protein; subregion II, which is important for binding of the Rep protein and for initiation of DNA replication; and subregion III, which is important for DNA replication but apparently not for binding of the Rep protein. The Rep protein might recognize three specific DNA elements in subregions I and II. The relative transformation frequency of the autonomously replicating plasmids carrying deletions in subregion I is lower, and nevertheless the copy numbers of these plasmids in host bacteria are higher than those of the wild-type plasmid. Efficient and stable binding of the Rep protein to the origin might be important for the replication efficiency to be at the normal (low) level. Subregion II might be essential for interaction with the catalytic domain of the Rep protein for primer RNA synthesis. The 8-bp sequence across the border of subregions II and III, including the primer sequence, is conserved in the (putative) origins of many plasmids, the putative Rep proteins of which are related to the ColE2-P9 Rep protein. Subregion III might be required for a step that is necessary after Rep protein binding has taken place.
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