ArticleJournal of bacteriology2006
GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Natural tristability of a confined helical filament with anisotropic bending rigidities.Scientific reports · 2024Article
- Article
- Mechanical property of the helical configuration for a twisted intrinsically straight biopolymer.European biophysics journal : EBJ · 2019Article
- Conservation of conformational dynamics across prokaryotic actins.PLoS computational biology · 2019Article
- Effect of A22 on the Conformation of Bacterial Actin MreB.International journal of molecular sciences · 2019Article
- Article
- Status of Targeting MreB for the Development of Antibiotics.Frontiers in chemistry · 2019Review
- A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.PLoS genetics · 2017Article
- Bacterial Filament Systems: Toward Understanding Their Emergent Behavior and Cellular Functions.The Journal of biological chemistry · 2015Review
- Molecular mechanisms for the evolution of bacterial morphologies and growth modes.Frontiers in microbiology · 2015Review
- Article
- Effects of polymerization and nucleotide identity on the conformational dynamics of the bacterial actin homolog MreB.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?Biophysical journal · 2013Article
- The bacterial actin MamK: in vitro assembly behavior and filament architecture.The Journal of biological chemistry · 2013Article
- Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.Physiological reviews · 2012Review
- Cytoskeletal proteins of actinobacteria.International journal of cell biology · 2012Article
- Insight into the assembly properties and functional organisation of the magnetotactic bacterial actin-like homolog, MamK.PloS one · 2012Article
- Mutations in the nucleotide binding pocket of MreB can alter cell curvature and polar morphology in Caulobacter.Molecular microbiology · 2011Article
- Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.The Journal of biological chemistry · 2011Article
- The structure and function of bacterial actin homologs.Cold Spring Harbor perspectives in biology · 2010Review
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Authors and funding
3 authors.
Funding
Abstract
MreB, a major component of the recently discovered bacterial cytoskeleton, displays a structure homologous to its eukaryotic counterpart actin. Here, we study the assembly and mechanical properties of Thermotoga maritima MreB in the presence of different nucleotides in vitro. We found that GTP, not ADP or GDP, can mediate MreB assembly into filamentous structures as effectively as ATP. Upon MreB assembly, both GTP and ATP release the gamma phosphate at similar rates. Therefore, MreB is an equally effective ATPase and GTPase. Electron microscopy and quantitative rheology suggest that the morphologies and micromechanical properties of filamentous ATP-MreB and GTP-MreB are similar. In contrast, mammalian actin assembly is favored in the presence of ATP over GTP. These results indicate that, despite high structural homology of their monomers, T. maritima MreB and actin filaments display different assembly, morphology, micromechanics, and nucleotide-binding specificity. Furthermore, the biophysical properties of T. maritima MreB filaments, including high rigidity and propensity to form bundles, suggest a mechanism by which MreB helical structure may be involved in imposing a cylindrical architecture on rod-shaped bacterial cells.
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Registered trials
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