ArticleJournal of bacteriology2006
Function and evolution of plasmid-borne genes for pyrimidine biosynthesis in Borrelia spp.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Comparative genomics of Borrelia lusitaniae.G3 (Bethesda, Md.) · 2026Article
- Exploring microbial diversity and biosynthetic potential in zoo and wildlife animal microbiomes.Nature communications · 2024Article
- Article
- Functional Prokaryotic-Like Deoxycytidine Triphosphate Deaminases and Thymidylate Synthase in Eukaryotic Social Amoebae: Vertical, Endosymbiotic, or Horizontal Gene Transfer?Molecular biology and evolution · 2023Article
- Characterisation of aneLife · 2023Article
- Pathogenesis of Relapsing Fever.Current issues in molecular biology · 2021Review
- Multiple and Diverse vsp and vlp Sequences in Borrelia miyamotoi, a Hard Tick-Borne Zoonotic Pathogen.PloS one · 2016Article
- Phylogeny of a relapsing fever Borrelia species transmitted by the hard tick Ixodes scapularis.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2014Article
- Fibronectin-binding protein of Borrelia hermsii expressed in the blood of mice with relapsing fever.Infection and immunity · 2014Article
- Large linear plasmids of Borrelia species that cause relapsing fever.Journal of bacteriology · 2013Article
- Alp, an arthropod-associated outer membrane protein of Borrelia species that cause relapsing fever.Infection and immunity · 2012Article
- Downward causation by information control in micro-organisms.Interface focus · 2012Article
- Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids.PloS one · 2012Article
- GuaA and GuaB are essential for Borrelia burgdorferi survival in the tick-mouse infection cycle.Journal of bacteriology · 2009Article
- The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii.PLoS genetics · 2008Article
- Flavin-dependent thymidylate synthase X limits chromosomal DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Flavin-dependent thymidylate synthase ThyX activity: implications for the folate cycle in bacteria.Journal of bacteriology · 2007Article
- Purine salvage pathways among Borrelia species.Infection and immunity · 2007Article
- Antigenic variation by Borrelia hermsii occurs through recombination between extragenic repetitive elements on linear plasmids.Molecular microbiology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The thyX gene for thymidylate synthase of the Lyme borreliosis (LB) agent Borrelia burgdorferi is located in a 54-kb linear plasmid. In the present study, we identified an orthologous thymidylate synthase gene in the relapsing fever (RF) agent Borrelia hermsii, located it in a 180-kb linear plasmid, and demonstrated its expression. The functions of the B. hermsii and B. burgdorferi thyX gene products were evaluated both in vivo, by complementation of a thymidylate synthase-deficient Escherichia coli mutant, and in vitro, by testing their activities after purification. The B. hermsii thyX gene complemented the thyA mutation in E. coli, and purified B. hermsii ThyX protein catalyzed the conversion of dTMP from dUMP. In contrast, the B. burgdorferi ThyX protein had only weakly detectable activity in vitro, and the B. burgdorferi thyX gene did not provide complementation in vivo. The lack of activity of B. burgdorferi's ThyX protein was associated with the substitution of a cysteine for a highly conserved arginine at position 91. The B. hermsii thyX locus was further distinguished by the downstream presence in the plasmid of orthologues of nrdI, nrdE, and nrdF, which encode the subunits of ribonucleoside diphosphate reductase and which are not present in the LB agents B. burgdorferi and Borrelia garinii. Phylogenetic analysis suggested that the nrdIEF cluster of B. hermsii was acquired by horizontal gene transfer. These findings indicate that Borrelia spp. causing RF have a greater capability for de novo pyrimidine synthesis than those causing LB, thus providing a basis for some of the biological differences between the two groups of pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.