ArticleJournal of bacteriology2006
Topology and boundaries of the aerotaxis receptor Aer in the membrane of Escherichia coli.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Signaling mechanism of the transmembrane energy receptor Aer.bioRxiv : the preprint server for biology · 2026Article
- Mobility of Lower MA-Helices for Ion Conduction through Lateral Portals in 5-HTBiophysical journal · 2020Article
- Aer Receptors Influence theFrontiers in microbiology · 2020Article
- Methyl-accepting chemotaxis proteins: a core sensing element in prokaryotes and archaea.Cellular and molecular life sciences : CMLS · 2017Review
- Bacterial Energy Sensor Aer Modulates the Activity of the Chemotaxis Kinase CheA Based on the Redox State of the Flavin Cofactor.The Journal of biological chemistry · 2016Article
- Delineating PAS-HAMP interaction surfaces and signalling-associated changes in the aerotaxis receptor Aer.Molecular microbiology · 2016Article
- Different conformations of the kinase-on and kinase-off signaling states in the Aer HAMP domain.Journal of bacteriology · 2011Article
- Role of the F1 region in the Escherichia coli aerotaxis receptor Aer.Journal of bacteriology · 2011Article
- Gain-of-function mutations cluster in distinct regions associated with the signalling pathway in the PAS domain of the aerotaxis receptor, Aer.Molecular microbiology · 2010Article
- Bacterial energy taxis: a global strategy?Archives of microbiology · 2010Review
- Cellular localization of predicted transmembrane and soluble chemoreceptors in Sinorhizobium meliloti.Journal of bacteriology · 2009Article
- Characterization of CetA and CetB, a bipartite energy taxis system in Campylobacter jejuni.Molecular microbiology · 2008Article
- Structure-function relationships in the HAMP and proximal signaling domains of the aerotaxis receptor Aer.Journal of bacteriology · 2008Article
- Organization of the aerotaxis receptor aer in the membrane of Escherichia coli.Journal of bacteriology · 2007Article
- Aer on the inside looking out: paradigm for a PAS-HAMP role in sensing oxygen, redox and energy.Molecular microbiology · 2007Review
- Differentiation between electron transport sensing and proton motive force sensing by the Aer and Tsr receptors for aerotaxis.Molecular microbiology · 2006Article
- Loss- and gain-of-function mutations in the F1-HAMP region of the Escherichia coli aerotaxis transducer Aer.Journal of bacteriology · 2006Article
- Signaling interactions between the aerotaxis transducer Aer and heterologous chemoreceptors in Escherichia coli.Journal of bacteriology · 2006Article
- Function of the N-terminal cap of the PAS domain in signaling by the aerotaxis receptor Aer.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli chemoreceptors are type I membrane receptors that have a periplasmic sensing domain, a cytosolic signaling domain, and two transmembrane segments. The aerotaxis receptor, Aer, is different in that both its sensing and signaling regions are proposed to be cytosolic. This receptor has a 38-residue hydrophobic segment that is thought to form a membrane anchor. Most transmembrane prediction programs predict a single transmembrane-spanning segment, but such a topology is inconsistent with recent studies indicating that there is direct communication between the membrane flanking PAS and HAMP domains. We studied the overall topology and membrane boundaries of the Aer membrane anchor by a cysteine-scanning approach. The proximity of 48 cognate cysteine replacements in Aer dimers was determined in vivo by measuring the rate and extent of disulfide cross-linking after adding the oxidant copper phenanthroline, both at room temperature and to decrease lateral diffusion in the membrane, at 4 degrees C. Membrane boundaries were identified in membrane vesicles using 5-iodoacetamidofluorescein and methoxy polyethylene glycol 5000 (mPEG). To map periplasmic residues, accessible cysteines were blocked in whole cells by pretreatment with 4-acetamido-4'-maleimidylstilbene-2, 2' disulfonic acid before the cells were lysed in the presence of mPEG. The data were consistent with two membrane-spanning segments, separated by a short periplasmic loop. Although the membrane anchor contains a central proline residue that reaches the periplasm, its position was permissive to several amino acid and peptide replacements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.