ArticleBMC developmental biology2006
RCAS-RNAi: a loss-of-function method for the developing chick retina.
Article in BMC developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- AAV-mediated transduction of songbird retina.Frontiers in physiology · 2025Article
- Essential Role of BMP4 Signaling in the Avian Ceca in Colorectal Enteric Nervous System Development.International journal of molecular sciences · 2023Article
- Avian Adeno-Associated Viral Transduction of the Postembryonic Chicken Retina.Translational vision science & technology · 2019Article
- The chick eye in vision research: An excellent model for the study of ocular disease.Progress in retinal and eye research · 2017Review
- Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model.Journal of visualized experiments : JoVE · 2017Article
- Fgf8 Expression and Degradation of Retinoic Acid Are Required for Patterning a High-Acuity Area in the Retina.Developmental cell · 2017Article
- Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities.Scientific reports · 2017Article
- Pannexin 3 is required for late stage bone growth but not for initiation of ossification in avian embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Post-transcriptional regulation of satellite cell quiescence by TTP-mediated mRNA decay.eLife · 2015Article
- Neuroanatomy goes viral!Frontiers in neuroanatomy · 2015Review
- Use of lentiviral vectors to deliver and express bicistronic transgenes in developing chicken embryos.Methods (San Diego, Calif.) · 2014Article
- Dkk2/Frzb in the dermal papillae regulates feather regeneration.Developmental biology · 2014Article
- Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development.Molecular biology of the cell · 2014Article
- Ex vivo electroporation of retinal cells: a novel, high efficiency method for functional studies in primary retinal cultures.Experimental eye research · 2013Article
- Rediscovering the chick embryo as a model to study retinal development.Neural development · 2012Review
- A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression.Nature · 2011Article
- Mutations in the RNA granule component TDRD7 cause cataract and glaucoma.Science (New York, N.Y.) · 2011Article
- Wdr5 is required for chick skeletal development.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2010Article
- In vivo RNAi: today and tomorrow.Cold Spring Harbor perspectives in biology · 2010Review
- Intermuscular tendons are essential for the development of vertebrate stomach.Development (Cambridge, England) · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function.
resultsWe show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection.
conclusionThis technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.