ArticleClinical and vaccine immunology : CVI2006
Epithelial toll-like receptor 5 is constitutively localized in the mouse cecum and exhibits distinctive down-regulation during experimental colitis.
Article in Clinical and vaccine immunology : CVI, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Composition of Human-Associated Gut Microbiota Determines 3-DF and 3-HF Anti-Colitic Activity in IL-10 -/- Mice.Nutrients · 2024Article
- Characterization of the Molecular Events Underlying the Establishment of Axillary Meristem Region in Pepper.International journal of molecular sciences · 2023Article
- Necrotizing enterocolitis in premature infants-A defect in the brakes? Evidence from clinical and animal studies.Mucosal immunology · 2023Review
- Bacterial-Driven Inflammation and MutantCancer discovery · 2021Article
- Epithelial Toll-like receptors and their role in gut homeostasis and disease.Nature reviews. Gastroenterology & hepatology · 2020Review
- Interleukin-33 delays recovery of mucosal inflammation via downregulation of homeostatic ABCG5/8 in the colon.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- DNA methyltransferase inhibition reduces inflammation-induced colon tumorigenesis.Epigenetics · 2019Article
- Nutrients Mediate Intestinal Bacteria-Mucosal Immune Crosstalk.Frontiers in immunology · 2018Review
- Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors.Cancer & metabolism · 2018Article
- Transcriptional modulation of pattern recognition receptors in chronic colitis in mice is accompanied with Th1 and Th17 response.Biochemistry and biophysics reports · 2017Article
- Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world.Biochemistry and biophysics reports · 2017Review
- Mismatch Repair Proteins Initiate Epigenetic Alterations during Inflammation-Driven Tumorigenesis.Cancer research · 2017Article
- Physiological TLR5 expression in the intestine is regulated by differential DNA binding of Sp1/Sp3 through simultaneous Sp1 dephosphorylation and Sp3 phosphorylation by two different PKC isoforms.Nucleic acids research · 2016Article
- Compartmentalizing intestinal epithelial cell toll-like receptors for immune surveillance.Cellular and molecular life sciences : CMLS · 2015Review
- Reevaluating the hype: four bacterial metabolites under scrutiny.European journal of microbiology & immunology · 2015Review
- Toll-like receptor of mud crab, Scylla serrata: molecular characterisation, ontogeny and functional expression analysis following ligand exposure, and bacterial and viral infections.Molecular biology reports · 2014Article
- Ingestion of bacterial lipopolysaccharide inhibits peripheral taste responses to sucrose in mice.Neuroscience · 2014Article
- Host-microbial interactions and regulation of intestinal epithelial barrier function: From physiology to pathology.World journal of gastrointestinal pathophysiology · 2012Article
- TLR5 risk-associated haplotype for canine inflammatory bowel disease confers hyper-responsiveness to flagellin.PloS one · 2012Article
- Oxidative damage targets complexes containing DNA methyltransferases, SIRT1, and polycomb members to promoter CpG Islands.Cancer cell · 2011Article
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently demonstrated that the pattern recognition receptors (PRRs) toll-like receptor 2 (TLR2), TLR4, and CD14 are expressed in mouse colonic epithelium in a compartmentalized manner. Here we report the localization of TLR5, the receptor for bacterial flagellin, and its distinctive down-regulation during experimental colitis. Guts from normal BALB/c mice and those with dextran sodium sulfate (DSS)-induced colitis were compared. Each gut was divided into seven segments (stomach, small intestine [three parts], and colon [three parts]), and epithelial cells and crypt units were collected by scraping and EDTA treatment, respectively. Northern blotting showed that TLR5 mRNA was preferentially expressed in the epithelium of the proximal colon in normal mice. Laser capture microdissection coupled to reverse transcriptase PCR confirmed this localization. TLR5 protein expression reflected mRNA expression, as evidenced by Western blotting. In mice with acute colitis, inflammation occurred mainly in the distal colon. Interestingly, while TLR2, TLR4, and CD14 were up-regulated in the inflamed colon, TLR5 was down-regulated at both the mRNA and protein levels. Decreased TLR5 expression was more evident during chronic colitis. Additional in vitro studies using a mouse cell line, Colon-26, showed that gamma interferon (IFN-gamma) time- and dose-dependently down-regulates TLR5. In conclusion, epithelial cells, mainly in the proximal colon, constitutively express TLR5. TLR5 expression is down-regulated in vivo during acute and chronic DSS-induced colitis, in contrast to the expression of TLR2, TLR4, and CD14. The mechanism governing TLR5 regulation may therefore differ from that controlling other PRRs. Finally, IFN-gamma may be involved in down-regulating TLR5 expression.
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